AG08316
Fibroblast from Lung, Lung
Description:
MACACA MULATTA
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Nonhuman Primates
Animals |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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42,XX,abnormal 15
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Species
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Macaca mulatta
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Common Name
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rhesus monkey
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Remarks
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| PDL at Senescence |
83 |
| PDL at Freeze |
3 |
| Passage Frozen |
1 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Remarks |
The age of the donor is between 1 and 2 years. The biopsy was taken postmortem from the apex of the left lung on 7/9/85. A matching aortic smooth muscle cell culture from this monkey is AG08318. |
| Inagaki H, Ohye T, Kogo H, Yamada K, Kowa H, Shaikh TH, Emanuel BS, Kurahashi H, Palindromic AT-rich repeat in the NF1 gene is hypervariable in humans and evolutionarily conserved in primates Human mutation26:332-42 2005 |
| PubMed ID: 16116616 |
| PDL at Senescence |
83 |
| Cumulative PDL at Freeze |
3 |
| Passage Frozen |
1 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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