AG07921
Fibroblast from Skin, Arm
Description:
MACACA
ORDER: PRIMATES
NIA AGING CELL REPOSITORY DNA PANEL - PRIMATE PANEL: YOUNG AND AGED FOR EACH SPECIES REPRESENTED
Repository
|
NIA Aging Cell Culture Repository
|
Permit Requirements
|
CITES PERMIT REQUIRED IF SHIPPING OUTSIDE OF USA
|
Subcollection |
Animals |
Biopsy Source
|
Arm
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
42,XY
|
Species
|
Macaca nemestrina
|
Common Name
|
pigtailed macaque
|
Remarks
|
|
PDL at Senescence |
12 |
PDL at Freeze |
5 |
Passage Frozen |
2 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Genbank |
COX1 |
Remark |
The skin biopsy was taken ante-mortem on 11/14/84 from the inner aspect of the upper left arm. The culture was initiated using explants of minced skin tissue. Cell morphology is fibroblast-like. The karyotype is 42,XY; normal diploid male. |
Lo WS, Xu Z, Yu Z, Pun FW, Ng SK, Chen J, Tong KL, Zhao C, Xu X, Tsang SY, Harano M, Stöber G, Nimgaonkar VL, Xue H, Positive selection within the Schizophrenia-associated GABA(A) receptor beta(2) gene PloS one2:e462 2007 |
PubMed ID: 17520021 |
PDL at Senescence |
12 |
Cumulative PDL at Freeze |
5 |
Passage Frozen |
2 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
|
|