Repository NIA Aging Cell Culture Repository
Subcollection Canadian Alzheimer Disease
Biopsy Source Arm
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Race Caucasian
Ethnicity CANADIAN
Family Member 63
Relation to Proband VIII-56
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks The donor is 50% at risk for Alzheimer's disease. The skin biopsy was taken ante-mortem from the forearm. Culture was initiated on 12/10/84 using explants of minced skin. The cell morphology is fibroblast-like. Culture was frozen at PDL 5. A lymphoblast culture from same donor is AG07646.
PDL at Freeze 5
Passage Frozen 4
 
Remark The donor is 50% at risk for Alzheimer's disease. The skin biopsy was taken ante-mortem from the forearm. Culture was initiated on 12/10/84 using explants of minced skin. The cell morphology is fibroblast-like. Culture was frozen at PDL 5. A lymphoblast culture from same donor is AG07646.
St George-Hyslop PH, Tanzi RE, Polinsky RJ, Haines JL, Nee L, Watkins PC, Myers RH, Feldman RG, Pollen D, Drachman D, et al, The genetic defect causing familial Alzheimer's disease maps on chromosome 21. Science235:885-90 1987
PubMed ID: 2880399
 
Nee LE, Polinsky RJ, Eldridge R, Weingartner H, Smallberg S, Ebert M, A family with histologically confirmed Alzheimer's disease. Arch Neurol40:203-8 1983
PubMed ID: 6600923
No data is available
Cumulative PDL at Freeze 5
Passage Frozen 4
Split Ratio 1:2
Temperature 37 C
Percent CO2 5%
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA