AG07438
Fibroblast from Skin, Thorax
Description:
TRISOMY 21
NIA AGING CELL REPOSITORY DNA PANEL - DOWN SYNDROME
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Chromosome Abnormalities Heritable Diseases |
Biopsy Source
|
Thorax
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Race
|
Black/African American
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
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ISCN
|
47,XY,+21
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Freeze |
10 |
Passage Frozen |
7 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Remark |
The donor was diagnosed as Down syndrome (trisomy 21) and displayed typical facies. The skin biopsy was taken post-mortem on 3/15/84 and frozen. The culture was initiated on 06/7/84 using explants of minced skin tissue. The cell morphology is fibroblast-like. The karyotype is 47,XY,+21. |
Chen JY, Lin JR, Tsai FC, Meyer T., Dosage of Dyrk1a Shifts Cells within a p21-Cyclin D1 Signaling Map to Control the Decision to Enter the Cell Cycle. Mol Cell.52(1):87-100 2013 |
PubMed ID: 24119401 |
|
Kinde I, Papadopoulos N, Kinzler KW, Vogelstein B, FAST-SeqS: a simple and efficient method for the detection of aneuploidy by massively parallel sequencing PloS one7:e41162 2012 |
PubMed ID: 22815955 |
|
Prandini P, Deutsch S, Lyle R, Gagnebin M, Vivier CD, Delorenzi M, Gehrig C, Descombes P, Sherman S, Bricarelli FD, Baldo C, Novelli A, Dallapiccola B, Antonarakis SE, Natural gene-expression variation in down syndrome modulates the outcome of gene-dosage imbalance American journal of human genetics81:252-63 2007 |
PubMed ID: 17668376 |
Cumulative PDL at Freeze |
10 |
Passage Frozen |
7 |
Split Ratio |
1:2 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
|
|