AG07109
Fibroblast from Skin, Arm
Description:
MACACA MULATTA
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Nonhuman Primates
Animals |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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42,XX
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Species
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Macaca mulatta
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Common Name
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rhesus monkey
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Remarks
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| PDL at Senescence |
10 |
| PDL at Freeze |
13 |
| Passage Frozen |
7 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
Plasminogen |
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| Genbank |
Apolipoprotein B |
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| Genbank |
Lipoprotein Lp(a) |
| Remarks |
Indian strain of rhesus macaque; the age of the donor is estimated. The donor was acquired as an adult at the Oregon Regional Primate Center. The age given is the sum of the years at the center plus 5 years (the minimum age for an adult rhesus monkey). The skin biopsy was obtained on 10/20/83, ante-mortem, from the inner aspect of the upper left arm. |
| Ray DA, Xing J, Hedges DJ, Hall MA, Laborde ME, Anders BA, White BR, Stoilova N, Fowlkes JD, Landry KE, Chemnick LG, Ryder OA, Batzer MA, Alu insertion loci and platyrrhine primate phylogeny. Mol Phylogenet Evol35(1):117-26 2005 |
| PubMed ID: 15737586 |
| PDL at Senescence |
10 |
| Cumulative PDL at Freeze |
13 |
| Passage Frozen |
7 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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