Repository NIA Aging Cell Culture Repository
Subcollection Specially Characterized Fibroblasts
Quantity 10 µg
Quantitation Method Please see our FAQ
Biopsy Source Lung
Cell Type Fibroblast
Tissue Type Lung
Transformant Untransformed
Race Caucasian
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 46,XY
Species Homo sapiens
Common Name Human
Remarks The MRC-5 cell line was developed in September 1966 from lung tissue taken from a 14 week fetus aborted for psychiatric reason from a 27 year old physically healthy woman. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. Cumulative population doublings to senescence is 42-48. G6PD isoenzyme is type B.
PDL at Senescence 73
PDL at Freeze 27
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Chromosome Analysis
 
Remark The MRC-5 cell line was developed in September 1966 from lung tissue taken from a 14 week fetus aborted for psychiatric reason from a 27 year old physically healthy woman. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. Cumulative population doublings to senescence is 42-48. G6PD isoenzyme is type B.
Guo WJ, Datta S, Band V, Dimri GP, Mel-18, a polycomb group protein, regulates cell proliferation and senescence via transcriptional repression of Bmi-1 and c-Myc oncoproteins Molecular biology of the cell18:536-46 2006
PubMed ID: 17151361
 
Von Kobbe C, May A, Grandori C, Bohr VA, Werner syndrome cells escape hydrogen peroxide-induced cell proliferation arrest. FASEB J18(15):1970-2 2004
PubMed ID: 15459124
 
Skandalis A, Uribe E, A survey of splice variants of the human hypoxanthine phosphoribosyl transferase and DNA polymerase beta genes: products of alternative or aberrant splicing? Nucleic Acids Res32(22):6557-64 2004
PubMed ID: 15601998
 
Hande MP, Balajee AS, Tchirkov A, Wynshaw-Boris A, Lansdorp PM, Extra-chromosomal telomeric DNA in cells from Atm(-/-) mice and patients with ataxia-telangiectasia. Hum Mol Genet10(5):519-28 2001
PubMed ID: 11181576
 
Gyulai Z, Endresz V, Burian K, Pincus S, Toldy J, Cox WI, Meric C, Plotkin S, Gonczol E, Berencsi K, Cytotoxic T lymphocyte (CTL) responses to human cytomegalovirus pp65, IE1-Exon4, gB, pp150, and pp28 in healthy individuals: reevaluation of prevalence of IE1-specific CTLs. J Infect Dis181(5):1537-46 2000
PubMed ID: 10823751
 
Piret B, Schoonbroodt S, Piette J, The ATM protein is required for sustained activation of NF-kappaB following DNA damage. Oncogene18(13):2261-71 1999
PubMed ID: 10327072
 
Jongmans W, Vuillaume M, Chrzanowska K, Smeets D, Sperling K, Hall J, Nijmegen breakage syndrome cells fail to induce the p53-mediated DNA damage response following exposure to ionizing radiation. Mol Cell Biol17(9):5016-22 1997
PubMed ID: 9271379
 
Borsi L, Balza E, Gaggero B, Allemanni G, Zardi L, The alternative splicing pattern of the tenascin-C pre-mRNA is controlled by the extracellular pH. J Biol Chem270:6243-5 1995
PubMed ID: 7534307
 
Duan C, Clemmons DR, Transcription factor AP-2 regulates human insulin-like growth factor binding protein-5 gene expression. J Biol Chem270:24844-51 1995
PubMed ID: 7559606
 
Futreal PA, Cochran C, Marks JR, Iglehart JD, Zimmerman W, Barrett JC, Wiseman RW, Mutation analysis of the THRA1 gene in breast cancer: deletion/fusion of the gene to a novel sequence on 17q in the BT474 cell line. Cancer Res54:1791-4 1994
PubMed ID: 7511052
 
Kilfeather SA, Collins D, McCormack P, Cotter T, O'Malley K, The effect of in vitro aging on human lung fibroblast beta-adrenergic receptor density, coupling and response. Mech Ageing Dev63:247-56 1992
PubMed ID: 1319530
 
Jacobs, Characteristics of a human diploid cell designated MRC-5. Nature277:168 (1970):247-56 1970
PubMed ID: 1319530
No data is available
PDL at Senescence 73
Cumulative PDL at Freeze 27
Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA