AG05308
Fibroblast from Skin, Thigh
Description:
SAGUINUS LABIATUS
ORDER: PRIMATES
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Nonhuman Primates Animals |
Biopsy Source
|
Thigh
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Thigh
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
45,X{8}/46,XX{92}
|
Species
|
Saguinus labiatus
|
Common Name
|
red-chested mustached tamarin
|
Remarks
|
|
PDL at Freeze |
16 |
Passage Frozen |
13 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
|
Rheinemann L, Downhour DM, Davenport KA, McKeown AN, Sundquist WI, Elde NC, Recurrent evolution of an inhibitor of ESCRT-dependent virus budding and LINE-1 retrotransposition in primates Current biology : CB32:1511-1522.e6 2021 |
PubMed ID: 35245459 |
|
Diehl WE, Stansell E, Kaiser SM, Emerman M, Hunter E, Identification of Post-entry Restrictions to Mason-Pfizer Monkey Virus Infection in New World Monkey Cells Journal of virology82(22):11140-50 2008 |
PubMed ID: 18799582 |
|
Ray DA, Xing J, Hedges DJ, Hall MA, Laborde ME, Anders BA, White BR, Stoilova N, Fowlkes JD, Landry KE, Chemnick LG, Ryder OA, Batzer MA, Alu insertion loci and platyrrhine primate phylogeny. Mol Phylogenet Evol35(1):117-26 2005 |
PubMed ID: 15737586 |
|
Sawyer SL, Wu LI, Emerman M, Malik HS, Positive selection of primate TRIM5alpha identifies a critical species-specific retroviral restriction domain. Proc Natl Acad Sci U S A102(8):2832-7 2005 |
PubMed ID: 15689398 |
|
Song B, Gold B, O'Huigin C, Javanbakht H, Li X, Stremlau M, Winkler C, Dean M, Sodroski J, The B302(SPRY) domain of the retroviral restriction factor TRIM5alpha exhibits lineage-specific length and sequence variation in primates Journal of virology79:6111-21 2005 |
PubMed ID: 15857996 |
|
Nakamura KD, Hart RW, Comparison of proto oncogene expression in seven primate fibroblast cultures. Mech Ageing Dev39:177-87 1987 |
PubMed ID: 3626640 |
Cumulative PDL at Freeze |
16 |
Passage Frozen |
13 |
Split Ratio |
1:4 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
MEM (Eagle) Alpha Modification with nucleosides/Dulbecco Modified Eagles Medium (high glucose), 1:1 mixture with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|