AG05252
Fibroblast from Skin, Thigh
Description:
PONGO PYGMAEUS
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Nonhuman Primates
Animals |
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Biopsy Source
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Thigh
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Thigh
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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48,XY[44]/48,XY,t(6;13)(q22;q26)[6]
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Species
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Pongo pygmaeus
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Common Name
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Borneo orangutan
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Remarks
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|
| PDL at Freeze |
17 |
| Passage Frozen |
10 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Genbank |
COX1 |
| Remarks |
Bornean orangutan |
| Steinert S, White DM, Zou Y, Shay JW, Wright WE, Telomere biology and cellular aging in nonhuman primate cells. Exp Cell Res272(2):146-52 2002 |
| PubMed ID: 11777339 |
| |
| Conte RA,Samonte RV,Verma R, Localization of subtelomeric sequences of human chromosomes 1q, 11p, 13q, and 16q in the higher primates. Genetica105:31-4 1999 |
| PubMed ID: 10483091 |
| |
| Conte RA,Samonte RV,Verma R, Evolutionary divergence of the oncogenes GLI, HST and INT2. Heredity81 ( Pt 1):10-3 1998 |
| PubMed ID: 9720300 |
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| Kenyon L,Moraes C, Expanding the functional human mitochondrial DNA database by the establishment of primate xenomitochondrial cybrids. Proc Natl Acad Sci U S A94:9131-5 1997 |
| PubMed ID: 9256447 |
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| Tarzami ST,Kringstein AM,Conte RA,Verma R, Unique genomic sequences in human chromosome 16p are conserved in the great apes. Mol Gen Genet253:512-4 1997 |
| PubMed ID: 9037113 |
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| Ermert K,Mitlohner H,Schempp W,Zachau H, The immunoglobulin kappa locus of primates. Genomics25:623-9 1995 |
| PubMed ID: 7759095 |
| |
| Weber B, Weissenbach J, Schempp W, Conservation of human-derived pseudoautosomal sequences on the sex chromosomes of the great apes. Cytogenet Cell Genet45(1):26-9 1987 |
| PubMed ID: 3474106 |
| Cumulative PDL at Freeze |
17 |
| Passage Frozen |
10 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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