AG05120
Fibroblast from Umbilical Cord, Umbilical cord
Description:
TRISOMY 21
NIA AGING CELL REPOSITORY DNA PANEL - DOWN SYNDROME
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Chromosome Abnormalities Heritable Diseases |
Biopsy Source
|
Umbilical cord
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Umbilical Cord
|
Transformant
|
Untransformed
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
47,XX,+21
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Freeze |
9 |
Passage Frozen |
8 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Remark |
This culture was derived from minced umbilical cord tissue obtained from a therapeutically aborted fetus of 22 weeks gestational age. The karyotype is 47,XX,+21. The cell morphology is fibroblast-like. Culture was frozen at PDL 3. An amniotic fluid fibroblast culture established from same donor is AG05121. |
Kinde I, Papadopoulos N, Kinzler KW, Vogelstein B, FAST-SeqS: a simple and efficient method for the detection of aneuploidy by massively parallel sequencing PloS one7:e41162 2012 |
PubMed ID: 22815955 |
Cumulative PDL at Freeze |
9 |
Passage Frozen |
8 |
Split Ratio |
1:3 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
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