AG04676
Epithelial from Eye
Description:
ORYCTOLAGUS
ORDER: LAGOMORPHA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Eye
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Cell Type
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Epithelial
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Tissue Type
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Lens
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Transformant
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Untransformed
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Sample Source
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Epithelial from Eye
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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44,XX
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Species
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Oryctolagus cuniculus
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Common Name
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rabbit
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Remarks
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| PDL at Freeze |
112 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The culture was initiated on 9/2/79 from the ocular lens capsule of a New Zealand White rabbit. Cell morphology is epithelial-like. The karyotype is 44,XX; normal diploid female. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Hofmann W, Schubert D, LaBonte J, Munson L, Gibson S, Scammell J, Ferrigno P, Sodroski J, Species-specific, postentry barriers to primate immunodeficiency virus infection. J Virol73:10020-8 1999 |
| PubMed ID: 10559316 |
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| Megraud F, Gamon LB, McGarrity GJ, Characterization of Spiroplasma mirum (suckling mouse cataract agent) in a rabbit lens cell culture. Infect Immun42:1168-75 1983 |
| PubMed ID: 6642663 |
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| Reddan JR, Friedman TB, Mostafapour MK, Bondy RL, Sutherland SH, McGee SJ, Goldenberg EM, Donor age influences the growth of rabbit lens epithelial cells in vitro. Vision Res21:11-23 1981 |
| PubMed ID: 7269278 |
| Cumulative PDL at Freeze |
112 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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