AG04160
LCL from B-Lymphocyte
Description:
ALZHEIMER DISEASE, FAMILIAL, TYPE 3
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Canadian Alzheimer Disease |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Ethnicity
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CANADIAN
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Family Member
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44
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Relation to Proband
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VII-35
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Remarks |
The donor has a history of complex partial seizures for 40 years which have been controlled by anticonvulsants. Dementia beginning in early l970's has progressed with severe memory and language impairment. Neuro exam confirms severe dementia and regressive reflexes. CT scan shows generalized atrophy and ventricular dilatation. The culture was initiated on 6/24/80 by transformation of lymphocytes with Epstein Barr virus. The cells grow in suspension and their morphology is spherical. The karyotype is 46,XX; normal diploid female. A skin fibroblast culture from same donor is AG04159A. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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