Description:
HTT-Q82-PCDNA3.1, PURE, 1-3144 (S13A)
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Repository
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HD Community Biorepository
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| Quantity |
20 µg |
| Quantitation Method |
Please see our FAQ |
| Certain Third Party Licensing Requirements |
Invitrogen License for Cat# V790.20 |
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Alias
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Htt-Q82-pcDNA3.1, pure, 1-3144 (S13A)
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Family History
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N
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Remarks
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| Remarks |
DNA corresponding to full length human sequence of the Huntingtin gene (Htt) containing S13A mutation and 82 pure polyglutamine repeats in pcDNA3.1 |
| Construct Name |
Htt-Q82-pcDNA3.1, pure, 1-3144 (S13A) |
| Insert Length |
9.4 kb |
| Vector |
pcDNA3.1 |
| Concentration |
0.632 mg/ml |
| Sequence |
ATGGCGACCCTGGAAAAGCTGATGAAGGCCTTCGAGGCCCTCAAGTCCTTCCAGCAGCA
GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
AGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA
GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAAC
AGCCGCCACCGCCGCCGCCGCCGCCGCCGCCTCCTCAGCTTCCTCAGCCGCCGCCGCAG
GCACAGCCGCTGCTGCCTCAGCCGCAGCCGCCCCCGCCGCCGCCCCCGCCGCCACCCGG
CCCGGCTGTGGCTGAGGAGCCGCTGCACCGACCAAAGAAAGAACTTTCAGCTACCAAGA
AAGACCGTGTGAATCATTGTCTGACAATATGTGAAAACATAGTGGCACAGTCTGTCAGA
AATTCTCCAGAATTTCAGAAACTTCTGGGCATCGCTATGGAACTTTTTCTGCTGTGCAG
TGATGACGCAGAGTCAGATGTCAGGATGGTGGCTGACGAATGCCTCAACAAAGTTATCA
AAGCTTTGATGGATTCTAATCTTCCAAGGTTACAGCTCGAGCTCTACAAGGAGATTAAG
AAAAACGGCGCACCGCGCTCACTGCGCGCTGCTCTGTGGCGTTTTGCAGAACTCGCACA
CCTGGTGCGTCCTCAGAAATGTCGCCCGTACCTGGTGAACCTGCTGCCATGCCTCACCC
GTACCAGCAAGCGTCCGGAAGAATCCGTTCAGGAGACTCTGGCAGCGGCTGTGCCAAAG
ATCATGGCCTCATTCGGTAATTTTGCGAATGACAACGAGATTAAGGTGCTGCTGAAAGC
ATTCATTGCTAACCTGAAGTCATCTTCTCCAACCATCCGTCGCACTGCGGCGGGTTCCG
CGGTGAGCATCTGTCAGCACAGCCGCCGTACTCAGTACTTTTACTCATGGCTGCTGAAT
GTGCTGCTGGGTCTGCTGGTCCCAGTGGAGGATGAACACTCCACCCTGCTGATTCTCGG
CGTTCTGCTGACGCTGCGTTATCTGGTTCCGCTCCTGCAACAGCAGGTGAAGGACACCT
CACTGAAGGGCTCTTTTGGCGTTACTCGCAAAGAAATGGAGGTTTCCCCGTCCGCTGAG
CAGCTGGTGCAGGTCTATGAACTGACCCTGCACCACACTCAACATCAGGACCACAACGT
CGTCACCGGCGCACTGGAGCTGCTCCAGCAGCTGTTTCGCACGCCTCCTCCAGAGCTCC
TGCAGACTCTGACTGCCGTGGGTGGCATTGGCCAACTCACCGCTGCCAAAGAAGAATCT
GGCGGTCGCTCCCGTTCTGGCTCTATTGTGGAACTGATTGCGGGCGGTGGCAGCAGCTG
CAGCCCAGTCCTCTCCCGTAAGCAGAAAGGTAAAGTCCTGCTGGGCGAGGAAGAAGCCC
TGGAGGACGATTCCGAGTCCCGTTCCGACGTTTCCAGCAGCGCGCTGACCGCTTCAGTT
AAGGATGAGATTTCCGGTGAGCTGGCGGCTTCTTCTGGTGTGTCTACGCCTGGTTCAGC
CGGCCATGATATCATCACCGAACAGCCACGTTCACAGCACACCCTGCAGGCCGATAGCG
TGGACCTGGCTTCCTGCGATCTGACGTCCAGCGCAACCGACGGTGATGAAGAAGACATC
CTGAGCCACAGCTCTAGCCAAGTATCTGCCGTGCCTAGCGACCCTGCAATGGACCTGAA
TGACGGTACTCAAGCCTCTTCTCCGATTAGCGATTCTTCCCAGACCACTACCGAGGGCC
CAGACAGCGCTGTGACCCCAAGCGATTCCTCTGAAATTGTGCTGGACGGTACCGATAAC
CAGTACCTGGGTCTGCAGATTGGTCAACCTCAAGATGAAGACGAAGAGGCAACCGGTAT
TCTGCCGGATGAAGCGTCTGAGGCTTTCCGTAATAGCTCTATGGCCCTGCAGCAAGCAC
ATCTGCTGAAAAACATGTCCCACTGCCGTCAGCCATCCGACAGCTCTGTAGATAAATTC
GTCCTCCGTGACGAGGCTACCGAGCCTGGCGATCAAGAGAATAAACCTTGTCGCATTAA
AGGTGACATCGGTCAGAGCACCGACGATGACTCAGCTCCACTGGTGCATTGCGTGCGTC
TGCTGTCAGCTTCCTTTCTCCTGACCGGCGGTAAAAACGTACTGGTCCCTGACCGTGAC
GTGCGTGTATCCGTAAAAGCTCTGGCCCTGAGCTGCGTAGGTGCGGCCGTAGCCCTGCA
TCCTGAATCATTCTTCTCTAAACTGTACAAAGTACCACTGGACACTACCGAGTATCCGG
AAGAACAATACGTTTCTGATATTCTGAACTACATCGACCACGGTGACCCTCAGGTTCGT
GGCGCTACCGCAATCCTGTGCGGCACCCTGATCTGTAGCATTCTGTCACGCTCTCGTTT
CCACGTCGGCGATTGGATGGGTACTATCCGTACCCTGACCGGCAACACTTTTTCCCTGG
CGGACTGTATCCCACTGCTGCGCAAAACCCTGAAAGATGAGTCCAGCGTGACGTGCAAA
CTCGCCTGTACTGCCGTCCGTAATTGTGTCATGAGCCTGTGCAGCTCTTCCTATAGCGA
ACTGGGCCTGCAGCTCATCATCGATGTTCTGACTCTGCGTAACTCCTCCTATTGGCTCG
TTCGCACCGAGCTGCTCGAAACCCTGGCTGAAATCGACTTTCGCCTGGTGAGCTTCCTG
GAAGCCAAAGCCGAAAACCTGCATCGCGGTGCCCACCACTACACCGGTCTGCTGAAGCT
CCAGGAGCGCGTTCTGAACAACGTGGTGATCCACCTCCTGGGCGACGAAGACCCTCGTG
TTCGTCACGTTGCAGCCGCCTCTCTGATCCGTCTCGTACCTAAACTGTTCTACAAGTGC
GATCAGGGTCAAGCAGATCCAGTGGTGGCGGTGGCCCGCGACCAGTCCTCCGTTTATCT
GAAACTGCTGATGCACGAAACCCAGCCGCCTTCCCACTTCTCTGTGTCCACTATCACCC
GTATTTATCGCGGCTATAACCTCCTGCCGAGCATTACCGACGTAACGATGGAAAACAAC
CTGTCTCGCGTTATTGCCGCAGTTAGCCACGAGCTGATTACGAGCACGACCCGTGCCCT
GACTTTTGGCTGTTGTGAGGCCCTGTGCCTGCTGTCTACCGCGTTTCCAGTATGTATCT
GGAGCCTGGGTTGGCACTGTGGCGTCCCGCCTCTGAGCGCGTCTGACGAGAGCCGCAAG
TCTTGTACGGTGGGCATGGCCACCATGATCCTGACTCTCCTGTCCTCTGCCTGGTTTCC
ACTGGACCTGTCAGCGCACCAGGACGCACTGATTCTGGCAGGTAACCTGCTGGCAGCCT
CCGCTCCGAAAAGCCTGCGTTCTAGCTGGGCCTCAGAGGAGGAAGCTAATCCGGCAGCC
ACCAAACAGGAAGAAGTATGGCCTGCTCTGGGTGATCGCGCACTGGTTCCGATGGTTGA
ACAGCTCTTTTCACACCTGCTGAAAGTTATCAACATCTGTGCACACGTGCTGGACGACG
TCGCCCCTGGCCCTGCGATTAAAGCAGCGCTCCCGAGCCTGACCAATCCACCTTCTCTC
TCCCCTATTCGTCGTAAGGGCAAAGAAAAAGAACCGGGCGAACAGGCCAGCGTGCCTCT
CTCTCCTAAGAAAGGCAGCGAGGCTTCTGCCGCCAGCCGCCAGTCCGACACGTCCGGCC
CAGTCACCACCTCTAAATCCTCCAGCCTGGGCAGCTTCTATCACCTGCCTTCTTACCTG
AAGCTGCACGATGTGCTGAAGGCCACCCACGCGAATTATAAGGTGACCCTGGACCTGCA
GAATAGCACCGAAAAATTTGGTGGTTTCCTCCGCTCTGCCCTCGACGTGCTGTCCCAGA
TCCTGGAACTGGCCACCCTGCAGGATATTGGTAAATGTGTCGAGGAGATCCTGGGTTAC
CTGAAAAGCTGTTTTTCTCGTGAGCCGATGATGGCTACTGTCTGCGTTCAGCAACTGCT
GAAAACCCTGTTTGGTACTAATCTGGCTTCTCAGTTTGACGGCCTGTCCTCAAATCCAT
CTAAAAGCCAGGGTCGTGCACAGCGCCTGGGTTCAAGCTCAGTTCGCCCGGGTCTGTAT
CATTATTGCTTCATGGCGCCTTACACCCATTTTACCCAGGCGCTCGCGGACGCATCTCT
CCGTAACATGGTGCAGGCCGAACAGGAGAACGACACCTCCGGTTGGTTCGATGTCCTGC
AGAAAGTTTCTACTCAGCTGAAGACCAACCTGACCTCAGTTACCAAAAACCGTGCCGAC
AAAAACGCCATTCATAACCATATTCGCCTGTTTGAACCGCTGGTAATCAAGGCCCTGAA
ACAGTACACCACTACGACTTGCGTGCAACTGCAGAAGCAGGTTCTGGATCTGCTGGCTC
AGCTGGTCCAGCTGCGTGTGAACTACTGCCTGCTGGACTCAGATCAGGTTTTTATTGGC
TTTGTACTGAAACAGTTTGAGTATATCGAAGTGGGCCAGTTTCGTGAATCCGAAGCCAT
CATCCCTAATATCTTTTTCTTCCTGGTTCTGCTCTCCTACGAACGTTATCACAGCAAAC
AGATCATCGGCATCCCGAAGATCATCCAGCTGTGCGACGGCATCATGGCATCCGGCCGC
AAAGCCGTTACCCATGCCATCCCAGCTCTGCAGCCTATTGTTCACGACCTGTTTGTCCT
CCGTGGCACCAACAAGGCAGACGCCGGCAAGGAACTGGAAACGCAGAAGGAAGTTGTCG
TGTCTATGCTGCTCCGTCTGATCCAATACCACCAGGTGCTGGAGATGTTTATCCTGGTC
CTGCAACAGTGTCACAAGGAGAACGAGGACAAATGGAAACGCCTGTCTCGCCAGATCGC
GGACATTATTCTGCCAATGCTGGCAAAGCAGCAGATGCACATCGACTCCCACGAAGCGC
TGGGCGTGCTGAATACCCTGTTCGAGATCCTGGCGCCTTCATCCCTGCGTCCGGTGGAC
ATGCTGCTGCGTAGCATGTTCGTCACCCCGAACACCATGGCTAGCGTGAGCACCGTCCA
ACTGTGGATTAGCGGTATCCTCGCAATCCTGCGCGTTCTGATTTCACAGAGCACGGAAG
ATATTGTGCTGTCACGTATTCAGGAACTGAGCTTCTCCCCATATCTGATCAGCTGTACT
GTTATCAACCGTCTGCGTGACGGCGACTCTACGTCCACCCTGGAAGAGCACTCTGAAGG
TAAGCAGATTAAAAACCTGCCGGAGGAAACGTTCTCCCGCTTCCTCCTGCAGCTGGTAG
GTATTCTCCTGGAAGACATTGTGACTAAACAGCTGAAAGTTGAAATGAGCGAGCAGCAG
CACACTTTCTACTGTCAGGAGCTGGGTACTCTGCTGATGTGTCTGATCCACATCTTTAA
AAGCGGCATGTTCCGTCGTATCACCGCCGCAGCAACTCGTCTGTTTCGTAGCGACGGCT
GCGGCGGCTCTTTCTACACCCTGGACAGCCTCAACCTGCGTGCACGTTCCATGATCACC
ACTCACCCTGCGCTGGTGCTGCTGTGGTGCCAAATCCTCCTGCTGGTGAACCATACCGA
TTACCGTTGGTGGGCGGAAGTTCAGCAGACTCCTAAGCGTCACTCCCTCTCCAGCACCA
AACTGCTCAGCCCGCAAATGTCTGGCGAAGAAGAAGACTCCGACCTGGCCGCCAAGCTG
GGCATGTGCAACCGCGAAATTGTGCGTCGTGGTGCCCTGATCCTGTTCTGTGACTATGT
TTGTCAGAATCTGCACGACTCTGAGCATCTGACCTGGCTGATTGTGAATCACATCCAGG
ACCTCATTAGCCTGTCCCACGAACCACCGGTCCAGGACTTCATTAGCGCTGTGCATCGT
AACAGCGCAGCTTCAGGCCTGTTCATCCAAGCAATCCAGTCTCGTTGCGAAAACCTGTC
CACCCCGACCATGCTGAAGAAAACTCTGCAGTGTCTGGAGGGCATCCACCTGTCCCAAT
CCGGTGCGGTCCTGACCCTCTACGTTGACCGCCTGCTCTGCACCCCATTCCGTGTTCTC
GCCCGTATGGTGGACATCCTCGCCTGCCGTCGCGTGGAAATGCTCCTGGCTGCCAATCT
GCAGTCTTCAATGGCACAGCTGCCAATGGAAGAGCTGAACCGTATCCAGGAATACCTGC
AGTCTTCCGGTCTGGCGCAGCGCCATCAGCGCCTCTACTCTCTGCTCGACCGTTTCCGT
CTGTCTACTATGCAGGATAGCCTGAGCCCATCTCCGCCGGTGTCCTCTCACCCGCTGGA
CGGCGATGGTCACGTGAGCCTCGAAACGGTATCTCCGGACAAAGATTGGTATGTTCACC
TGGTGAAAAGCCAGTGCTGGACTCGCAGCGACTCCGCCCTGCTGGAAGGCGCAGAACTG
GTTAACCGCATTCCAGCGGAGGACATGAACGCATTCATGATGAATTCAGAATTCAACCT
GAGCCTGCTGGCTCCGTGCCTGTCCCTCGGTATGAGCGAAATCTCAGGCGGCCAAAAGA
GCGCGCTGTTCGAAGCAGCTCGCGAAGTGACCCTGGCGCGCGTTAGCGGCACTGTGCAG
CAGCTGCCGGCAGTGCACCATGTTTTCCAGCCGGAGCTGCCAGCGGAACCGGCTGCCTA
CTGGTCCAAGCTGAACGACCTGTTCGGCGACGCCGCACTCTATCAGAGCCTGCCTACTC
TGGCGCGTGCTCTGGCGCAGTATCTGGTAGTGGTGAGCAAACTGCCGTCTCATCTGCAC
CTGCCGCCTGAAAAAGAGAAGGACATCGTCAAATTTGTTGTTGCAACCCTGGAGGCCCT
GTCCTGGCATCTCATTCACGAGCAGATCCCTCTGTCTCTGGATCTGCAAGCTGGCCTGG
ACTGCTGCTGCCTGGCTCTGCAGCTGCCAGGCCTGTGGTCTGTCGTCTCTTCCACTGAA
TTTGTTACTCACGCTTGCAGCCTGATTTATTGCGTACACTTCATTCTGGAGGCTGTGGC
TGTCCAGCCGGGCGAGCAGCTGCTGTCTCCGGAGCGCCGTACCAATACTCCAAAAGCCA
TTAGCGAAGAAGAGGAAGAAGTAGACCCTAACACTCAGAATCCGAAATATATCACGGCA
GCGTGTGAAATGGTTGCTGAAATGGTAGAGTCCCTGCAATCTGTACTGGCGCTGGGCCA
TAAACGTAACAGCGGCGTTCCGGCATTCCTGACTCCACTGCTGCGTAACATCATCATCT
CCCTGGCTCGTCTGCCGCTGGTAAATAGCTATACCCGTGTTCCGCCGCTGGTGTGGAAA
CTGGGTTGGTCCCCAAAACCAGGTGGTGATTTCGGTACTGCATTCCCTGAGATCCCAGT
AGAGTTTCTGCAAGAAAAAGAAGTGTTCAAGGAATTCATCTACCGCATCAATACTCTGG
GCTGGACGTCCCGCACGCAGTTCGAAGAGACTTGGGCGACCCTGCTCGGCGTCCTGGTT
ACCCAACCTCTGGTAATGGAGCAGGAAGAATCCCCTCCAGAAGAAGACACCGAACGTAC
CCAGATTAACGTCCTGGCAGTGCAGGCCATCACCAGCCTGGTCCTGTCAGCTATGACCG
TGCCAGTGGCGGGTAACCCGGCTGTGTCCTGTCTGGAACAGCAGCCACGTAACAAACCT
CTGAAAGCACTGGACACGCGCTTCGGCCGTAAACTGTCCATCATCCGTGGCATCGTCGA
GCAGGAAATCCAGGCTATGGTTTCTAAGCGTGAGAACATTGCTACCCACCACCTGTACC
AGGCGTGGGATCCGGTCCCGTCTCTCAGCCCGGCTACTACTGGCGCGCTGATTAGCCAC
GAAAAACTGCTGCTGCAGATTAACCCAGAACGTGAACTCGGTTCTATGTCATATAAACT
GGGTCAAGTCAGCATCCACTCCGTTTGGCTCGGTAACTCTATCACCCCTCTGCGTGAGG
AGGAGTGGGACGAGGAAGAAGAAGAGGAGGCAGATGCGCCTGCACCGTCCTCTCCACCA
ACTTCCCCAGTGAACTCCCGCAAACACCGCGCAGGTGTCGATATTCACAGCTGCTCTCA
GTTCCTGCTGGAACTGTATTCCCGCTGGATCCTGCCGTCAAGCTCCGCACGTCGCACTC
CGGCGATTCTGATTAGCGAGGTGGTGCGCTCTCTGCTCGTTGTCTCTGATCTGTTTACC
GAGCGCAATCAGTTTGAACTGATGTATGTAACTCTGACTGAACTGCGCCGTGTGCATCC
GTCCGAAGACGAAATTCTGGCTCAGTACCTGGTCCCGGCTACCTGTAAGGCTGCTGCTG
TTCTGGGCATGGACAAGGCTGTGGCAGAACCGGTCAGCCGCCTGCTGGAAAGCACCCTG
CGTTCCTCCCACCTGCCATCACGCGTGGGTGCGCTGCACGGTGTCCTGTACGTACTGGA
ATGTGACCTGCTGGATGACACCGCTAAGCAACTGATTCCAGTGATTAGCGATTATCTGC
TGAGCAACCTGAAGGGCATCGCGCATTGTGTGAACATCCACTCTCAACAACATGTGCTC
GTGATGTGCGCCACCGCCTTCTACCTGATCGAGAACTATCCGCTGGATGTGGGTCCTGA
ATTCTCCGCCTCTATTATCCAGATGTGCGGCGTTATGCTGTCAGGCAGCGAAGAGTCTA
CGCCGTCCATCATCTATCACTGTGCACTGCGCGGTCTGGAGCGTCTCCTGCTGTCCGAA
CAGCTGTCTCGTCTGGACGCGGAGAGCCTGGTTAAACTCAGCGTAGACCGTGTTAATGT
TCACTCTCCGCATCGTGCTATGGCAGCACTGGGCCTCATGCTGACTTGCATGTATACCG
GTAAAGAGAAGGTGTCTCCGGGTCGCACTTCCGACCCAAATCCGGCCGCTCCTGACTCC
GAATCCGTCATTGTGGCCATGGAACGTGTTTCAGTGCTCTTTGACCGCATCCGCAAGGG
CTTCCCATGTGAGGCCCGCGTGGTTGCGCGCATCCTGCCGCAGTTTCTGGACGACTTTT
TCCCGCCACAGGACATTATGAACAAAGTTATCGGCGAATTCCTGTCCAACCAGCAGCCA
TACCCTCAGTTCATGGCGACCGTTGTCTATAAAGTATTCCAGACCCTCCATAGCACTGG
TCAATCATCCATGGTACGTGATTGGGTTATGCTGAGCCTGTCTAACTTCACCCAGCGCG
CTCCGGTGGCTATGGCAACCTGGTCACTGTCTTGCTTCTTCGTATCTGCTTCCACGTCA
CCTTGGGTGGCGGCGATCCTCCCTCACGTAATCAGCCGTATGGGTAAACTGGAACAGGT
GGATGTTAATCTCTTTTGTCTGGTCGCCACTGACTTCTATCGTCATCAGATCGAGGAAG
AGCTCGACCGCCGCGCATTCCAGTCTGTCCTGGAAGTAGTTGCCGCTCCGGGCTCACCG
TATCACCGTCTCCTGACCTGCCTGCGCAACGTCCACAAAGTGACCACTTGTTGA)
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| Cloning Information |
Mammalian expression construct encoding FL-Htt with PTM alteration was generated in two steps. As a first step, the alteration was introduced within an existing Htt-N586-82Q plasmid (obtained from D. Borchelt and described in Tebbenkamp et al, 2012), using site-directed mutagenesis and the QuikChange II XL kit (Stratagene) according to the manufacturer |
| Remarks |
DNA corresponding to full length human sequence of the Huntingtin gene (Htt) containing S13A mutation and 82 pure polyglutamine repeats in pcDNA3.1 |
| DNA corresponding to full length human sequence of the Huntingtin gene (Htt) containing S13A mutation and 82 pure polyglutamine repeats in pcDNA3.1 |
| Remarks |
DNA corresponding to full length human sequence of the Huntingtin gene (Htt) containing S13A mutation and 82 pure polyglutamine repeats in pcDNA3.1 |
| PDF |
Vector Map |
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Plasmid Collection Screening and Propagation |
| Supplement |
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