Description:
HTT-Q82-PCDNA3.1, PURE, 1-586 (K444R)
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Repository
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HD Community Biorepository
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| Quantity |
20 µg |
| Quantitation Method |
Please see our FAQ |
| Certain Third Party Licensing Requirements |
Invitrogen License for Cat# V790.20 |
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Alias
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Htt-Q82-pcDNA3.1, pure, 1-586 (K444R)
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Family History
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N
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Remarks
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| Remarks |
DNA corresponding to fragment 1-586 human sequence of the Huntingtin (Htt) containing K444R mutation and 82 pure polyglutamine repeats in pcDNA3.1 |
| Construct Name |
Htt-Q82-pcDNA3.1, pure, 1-586 (K444R) |
| Insert Length |
1.8 kb |
| Vector |
pcDNA3.1 |
| Concentration |
0.233 mg/ml |
| Sequence |
ATGGCGACCCTGGAAAAGCTGATGAAGGCCTTCGAGTCCCTCAAGTCCTTCCAGCAGCA
GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
AGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA
GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAAC
AGCCGCCACCGCCGCCGCCGCCGCCGCCGCCTCCTCAGCTTCCTCAGCCGCCGCCGCAG
GCACAGCCGCTGCTGCCTCAGCCGCAGCCGCCCCCGCCGCCGCCCCCGCCGCCACCCGG
CCCGGCTGTGGCTGAGGAGCCGCTGCACCGACCAAAGAAAGAACTTTCAGCTACCAAGA
AAGACCGTGTGAATCATTGTCTGACAATATGTGAAAACATAGTGGCACAGTCTGTCAGA
AATTCTCCAGAATTTCAGAAACTTCTGGGCATCGCTATGGAACTTTTTCTGCTGTGCAG
TGATGACGCAGAGTCAGATGTCAGGATGGTGGCTGACGAATGCCTCAACAAAGTTATCA
AAGCTTTGATGGATTCTAATCTTCCAAGGTTACAGCTCGAGCTCTATAAGGAAATTAAA
AAGAATGGTGCCCCTCGGAGTTTGCGTGCTGCCCTGTGGAGGTTTGCTGAGCTGGCTCA
CCTGGTTCGGCCTCAGAAATGCAGGCCTTACCTGGTGAACCTTCTGCCGTGCCTGACTC
GAACAAGCAAGAGACCCGAAGAATCAGTCCAGGAGACCTTGGCTGCAGCTGTTCCCAAA
ATTATGGCTTCTTTTGGCAATTTTGCAAATGACAATGAAATTAAGGTTTTGTTAAAGGC
CTTCATAGCGAACCTGAAGTCAAGCTCCCCCACCATTCGGCGGACAGCGGCTGGATCAG
CAGTGAGCATCTGCCAGCACTCAAGAAGGACACAATATTTCTATAGTTGGCTACTAAAT
GTGCTCTTAGGCTTACTCGTTCCTGTCGAGGATGAACACTCCACTCTGCTGATTCTTGG
CGTGCTGCTCACCCTGAGGTATTTGGTGCCCTTGCTGCAGCAGCAGGTCAAGGACACAA
GCCTGAAAGGCAGCTTCGGAGTGACAAGGAAAGAAATGGAAGTCTCTCCTTCTGCAGAG
CAGCTTGTCCAGGTTTATGAACTGACGTTACATCATACACAGCACCAAGACCACAATGT
TGTGACCGGAGCCCTGGAGCTGTTGCAGCAGCTCTTCAGAACGCCTCCACCCGAGCTTC
TGCAAACCCTGACCGCAGTCGGGGGCATTGGGCAGCTCACCGCTGCTAAGGAGGAGTCT
GGTGGCCGAAGCCGTAGTGGGAGTATTGTGGAACTTATAGCTGGAGGGGGTTCCTCATG
CAGCCCTGTCCTTTCAAGAAAACAAAAAGGCAGAGTGCTCTTAGGAGAAGAAGAAGCCT
TGGAGGATGACTCTGAATCGAGATCGGATGTCAGCAGCTCTGCCTTAACAGCCTCAGTG
AAGGATGAGATCAGTGGAGAGCTGGCTGCTTCTTCAGGGGTTTCCACTCCAGGGTCAGC
AGGTCATGACATCATCACAGAACAGCCACGGTCACAGCACACACTGCAGGCGGACTCAG
TGGATCTGGCCAGCTGTGACTTGACAAGCTCTGCCACTGATGGGGATGAAGAGGATATC
TTGAGCCACAGCTCCAGCCAGGTCAGCGCCGTCCCATCTGACCCTGCCATGGACCTGAA
TGATGGGACCCAGGCCTCGTCGCCCATCAGCGACAGCTCCCAGACCACCACCGAAGGGC
CTGATTCAGCTGTTACCCCTTCAGACAGTTCTGAAATTGTGTTAGACTAA
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| Cloning Information |
Alterations were introduced within an existing Htt-N586-82Q plasmid (obtained from D. Borchelt and described in Tebbenkamp et al, 2012), using site-directed mutagenesis and the QuikChange II XL kit (Stratagene) according to the manufacturer's protocol, except that Stbl2' competent cells (Life Technologies) specifically designed for cloning unstable inserts were used |
| Remarks |
DNA corresponding to fragment 1-586 human sequence of the Huntingtin (Htt) containing K444R mutation and 82 pure polyglutamine repeats in pcDNA3.1 |
| DNA corresponding to fragment 1-586 human sequence of the Huntingtin (Htt) containing K444R mutation and 82 pure polyglutamine repeats in pcDNA3.1 |
| Remarks |
DNA corresponding to fragment 1-586 human sequence of the Huntingtin (Htt) containing K444R mutation and 82 pure polyglutamine repeats in pcDNA3.1 |
| PDF |
Vector Map |
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Plasmid Collection Screening and Propagation |
| Supplement |
- |
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