Description:
HTT-Q82-PCDNA3.1, PURE, 1-586 (T271A)
|
Repository
|
HD Community Biorepository
|
| Quantity |
20 µg |
| Quantitation Method |
Please see our FAQ |
| Certain Third Party Licensing Requirements |
Invitrogen License for Cat# V790.20 |
|
Alias
|
Htt-Q82-pcDNA3.1, pure, 1-586 (T271A)
|
|
Family History
|
N
|
|
Remarks
|
|
| Remarks |
DNA corresponding to fragment 1-586 human sequence of the Huntingtin (Htt) containing T271A mutation and 82 pure polyglutamine repeats in pcDNA3.1 |
| Construct Name |
Htt-Q82-pcDNA3.1, pure, 1-586 (T271A) |
| Insert Length |
1.8 kb |
| Vector |
pcDNA3.1 |
| Concentration |
0.777 mg/ml |
| Sequence |
ATGGCGACCCTGGAAAAGCTGATGAAGGCCTTCGAGTCCCTCAAGTCCTTCCAGCAGCA
GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
AGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA
GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAAC
AGCCGCCACCGCCGCCGCCGCCGCCGCCGCCTCCTCAGCTACCTCAGCCGCCGCCGCAG
GCACAGCCGCTGCTGCCTCAGCCGCAGCCGCCCCcGCCGCcGCCCCCGCCGCCACCCGg
CCCGGCTGTGGCTGAGGAGCCGCTGCACCGACCAAAGAAAGAACTTTCAGCTACCAAGA
AAGACCGTGTGAATCATTGTCTGACAATATGTGAAAACATAGTGGCACAGTCTGTCAGA
AATTCTCCAGAATTTCAGAAACTTCTGGGCATCGCTATGGAACTTTTTCTGCTGTGCAG
TGATGACGCAGAGTCAGATGTCAGGATGGTGGCTGACGAATGCCTCAACAAAGTTATCA
AAGCTTTGATGGATTCTAATCTTCCAAGGTTACAGCTCGAGCTCTATAAGGAAATTAAA
AAGAATGGTGCCCCTCGGAGTTTGCGTGCTGCCCTGTGGAGGTTTGCTGAGCTGGCTCA
CCTGGTTCGGCCTCAGAAATGCAGGCCTTACCTGGTGAACCTTCTGCCGTGCCTGACTC
GAACAAGCAAGAGACCCGAAGAATCAGTCCAGGAGACCTTGGCTGCAGCTGTTCCCAAA
ATTATGGCTTCTTTTGGCAATTTTGCAAATGACAATGAAATTAAGGTTTTGTTAAAGGC
CTTCATAGCGAACCTGAAGTCAAGCTCCCCCACCATTCGGCGGGCAGCGGCTGGATCAG
CAGTGAGCATCTGCCAGCACTCAAGAAGGACACAATATTTCTATAGTTGGCTACTAAAT
GTGCTCTTAGGCTTACTCGTTCCTGTCGAGGATGAACACTCCACTCTGCTGATTCTTGG
CGTGCTGCTCACCCTGAGGTATTTGGTGCCCTTGCTGCAGCAGCAGGTCAAGGACACAA
GCCTGAAAGGCAGCTTCGGAGTGACAAGGAAAGAAATGGAAGTCTCTCCTTCTGCAGAG
CAGCTTGTCCAGGTTTATGAACTGACGTTACATCATACACAGCACCAAGACCACAATGT
TGTGACCGGAGCCCTGGAGCTGTTGCAGCAGCTCTTCAGAACGCCTCCACCCGAGCTTC
TGCAAACCCTGACCGCAGTCGGGGGCATTGGGCAGCTCACCGCTGCTAAGGAGGAGTCT
GGTGGCCGAAGCCGTAGTGGGAGTATTGTGGAACTTATAGCTGGAGGGGGTTCCTCATG
CAGCCCTGTCCTTTCAAGAAAACAAAAAGGCAAAGTGCTCTTAGGAGAAGAAGAAGCCT
TGGAGGATGACTCTGAATCGAGATCGGATGTCAGCAGCTCTGCCTTAACAGCCTCAGTG
AAGGATGAGATCAGTGGAGAGCTGGCTGCTTCTTCAGGGGTTTCCACTCCAGGGTCAGC
AGGTCATGACATCATCACAGAACAGCCACGGTCACAGCACACACTGCAGGCGGACTCAG
TGGATCTGGCCAGCTGTGACTTGACAAGCTCTGCCACTGATGGGGATGAGGAGGATATC
TTGAGCCACAGCTCCAGCCAGGTCAGCGCCGTCCCATCTGACCCTGCCATGGACCTGAA
TGATGGGACCCAGGCCTCGTCGCCCATCAGCGACAGCTCCCAGACCACCACCGAAGGGC
CTGATTCAGCTGTTACCCCTTCAGACAGTTCTGAAATTGTGtTAGACTAA
|
| Cloning Information |
Alterations were introduced within an existing Htt-N586-82Q plasmid (obtained from D. Borchelt and described in Tebbenkamp et al, 2012), using site-directed mutagenesis and the QuikChange II XL kit (Stratagene) according to the manufacturer's protocol |
| Remarks |
DNA corresponding to fragment 1-586 human sequence of the Huntingtin (Htt) containing T271A mutation and 82 pure polyglutamine repeats in pcDNA3.1 |
| DNA corresponding to fragment 1-586 human sequence of the Huntingtin (Htt) containing T271A mutation and 82 pure polyglutamine repeats in pcDNA3.1 |
| Remarks |
DNA corresponding to fragment 1-586 human sequence of the Huntingtin (Htt) containing T271A mutation and 82 pure polyglutamine repeats in pcDNA3.1 |
| PDF |
Vector Map |
|
Plasmid Collection Screening and Propagation |
| Supplement |
- |
|