Description:
HTT-Q81-PCDNA3.1, PURE, 1-586 (S13,16A)
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Repository
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HD Community Biorepository
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| Quantity |
20 µg |
| Quantitation Method |
Please see our FAQ |
| Certain Third Party Licensing Requirements |
Invitrogen License for Cat# V790.20 |
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Alias
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Htt-Q81-pcDNA3.1, pure, 1-586 (S13,16A)
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Family History
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N
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Remarks
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| Remarks |
DNA corresponding to fragment 1-586 human sequence of the Huntingtin (Htt) containing S13,16A mutation and 81 pure polyglutamine repeats in pcDNA3.1 |
| Construct Name |
Htt-Q81-pcDNA3.1, pure, 1-586 (S13,16A) |
| Insert Length |
1.8 kb |
| Vector |
pcDNA3.1 |
| Concentration |
0.322 mg/ml |
| Sequence |
ATGGCGACCCTGGAAAAGCTGATGAAGGCCTTCGAGGCCCTCAAGGCCTTCCAGCAGCA
GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
AGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAG
CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA
GCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAGC
CGCCACCGCCGCCGCCGCCGCCGCCGCCTCCTCAGCTTCCTCAGCCGCCGCCGCAGGCA
CAGCCGCTGCTGCCTCAGCCGCAGCCGCCCCCGCCGCCGCCCCCGCCGCCACCCGGCCC
GGCTGTGGCTGAGGAGCCGCTGCACCGACCAAAGAAAGAACTTTCAGCTACCAAGAAAG
ACCGTGTGAATCATTGTCTGACAATATGTGAAAACATAGTGGCACAGTCTGTCAGAAAT
TCTCCAGAATTTCAGAAACTTCTGGGCATCGCTATGGAACTTTTTCTGCTGTGCAGTGA
TGACGCAGAGTCAGATGTCAGGATGGTGGCTGACGAATGCCTCAACAAAGTTATCAAAG
CTTTGATGGATTCTAATCTTCCAAGGTTACAGCTCGAGCTCTATAAGGAAATTAAAAAG
AATGGTGCCCCTCGGAGTTTGCGTGCTGCCCTGTGGAGGTTTGCTGAGCTGGCTCACCT
GGTTCGGCCTCAGAAATGCAGGCCTTACCTGGTGAACCTTCTGCCGTGCCTGACTCGAA
CAAGCAAGAGACCCGAAGAATCAGTCCAGGAGACCTTGGCTGCAGCTGTTCCCAAAATT
ATGGCTTCTTTTGGCAATTTTGCAAATGACAATGAAATTAAGGTTTTGTTAAAGGCCTT
CATAGCGAACCTGAAGTCAAGCTCCCCCACCATTCGGCGGACAGCGGCTGGATCAGCAG
TGAGCATCTGCCAGCACTCAAGAAGGACACAATATTTCTATAGTTGGCTACTAAATGTG
CTCTTAGGCTTACTCGTTCCTGTCGAGGATGAACACTCCACTCTGCTGATTCTTGGCGT
GCTGCTCACCCTGAGGTATTTGGTGCCCTTGCTGCAGCAGCAGGTCAAGGACACAAGCC
TGAAAGGCAGCTTCGGAGTGACAAGGAAAGAAATGGAAGTCTCTCCTTCTGCAGAGCAG
CTTGTCCAGGTTTATGAACTGACGTTACATCATACACAGCACCAAGACCACAATGTTGT
GACCGGAGCCCTGGAGCTGTTGCAGCAGCTCTTCAGAACGCCTCCACCCGAGCTTCTGC
AAACCCTGACCGCAGTCGGGGGCATTGGGCAGCTCACCGCTGCTAAGGAGGAGTCTGGT
GGCCGAAGCCGTAGTGGGAGTATTGTGGAACTTATAGCTGGAGGGGGTTCCTCATGCAG
CCCTGTCCTTTCAAGAAAACAAAAAGGCAAAGTGCTCTTAGGAGAAGAAGAAGCCTTGG
AGGATGACTCTGAATCGAGATCGGATGTCAGCAGCTCTGCCTTAACAGCCTCAGTGAAG
GATGAGATCAGTGGAGAGCTGGCTGCTTCTTCAGGGGTTTCCACTCCAGGGTCAGCAGG
TCATGACATCATCACAGAACAGCCACGGTCACAGCACACACTGCAGGCGGACTCAGTGG
ATCTGGCCAGCTGTGACTTGACAAGCTCTGCCACTGATGGGGATGAGGAGGATATCTTG
AGCCACAGCTCCAGCCAGGTCAGCGCCGTCCCATCTGACCCTGCCATGGACCTGAATGA
TGGGACCCAGGCCTCGTCGCCCATCAGCGACAGCTCCCAGACCACCACCGAAGGGCCTG
ATTCAGCTGTTACCCCTTCAGACAGTTCTGAAATTGTGTTAGACTAA
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| Cloning Information |
Alterations were introduced within an existing Htt-N586-82Q plasmid (obtained from D. Borchelt and described in Tebbenkamp et al, 2012), using site-directed mutagenesis and the QuikChange II XL kit (Stratagene) according to the manufacturer's protocol, except that Stbl2' competent cells (Life Technologies) specifically designed for cloning unstable inserts were used |
| Remarks |
DNA corresponding to fragment 1-586 human sequence of the Huntingtin (Htt) containing S13,16A mutation and 81 pure polyglutamine repeats in pcDNA3.1 |
| DNA corresponding to fragment 1-586 human sequence of the Huntingtin (Htt) containing S13,16A mutation and 81 pure polyglutamine repeats in pcDNA3.1 |
| Remarks |
DNA corresponding to fragment 1-586 human sequence of the Huntingtin (Htt) containing S13,16A mutation and 81 pure polyglutamine repeats in pcDNA3.1 |
| PDF |
Vector Map |
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Plasmid Collection Screening and Propagation |
| Supplement |
- |
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