AG16557
Smooth muscle from Aortic arch
Description:
MACACA NEMESTRINA
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Nonhuman Primates
Animals |
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Biopsy Source
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Aortic arch
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Cell Type
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Smooth muscle
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Smooth muscle from Aortic arch
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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41,XX,-17[2]/42,XX[48]
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Species
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Macaca nemestrina
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Common Name
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pigtailed macaque
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Remarks
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| PDL at Senescence |
50 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Feral animal whose age was estimated. The donor was anemic at the time of death due to uterine bleeding. The biopsy was obtained post-mortem from the aortic arch. Skin fibroblast and aortic endothelial cell cultures from this same monkey are AG16550 and AG16551, respectively. |
| PDL at Senescence |
50 |
| Passage Frozen |
2 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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