AG14725
LCL from B-Lymphocyte
Description:
APPARENTLY HEALTHY INDIVIDUAL
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
"Intra-abdominal fat and risk of disease in children and adolescents" study Apparently Healthy Collection |
Biopsy Source
|
Peripheral vein
|
Cell Type
|
B-Lymphocyte
|
Tissue Type
|
Blood
|
Transformant
|
Epstein-Barr Virus
|
Sample Source
|
LCL from B-Lymphocyte
|
Race
|
White
|
Family Member
|
2
|
Relation to Proband
|
brother
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
46,XY
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
|
Remarks |
Participant in the "intra-abdominal fat and risk of disease in children and adolescents" study. The culture was initiated on 9/23/97 by transformation of lymphocytes with Epstein Barr virus. The culture grows in suspension and the cell morphology is spherical. The karyotype is 46,XY with 6% of the cells examined showing random chromosome loss and 2% showing random chromosomal abberations. A Lymphoblast culture from this donor's brother is AG14724. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
Goran MI, Metabolic precursors and effects of obesity in children: a decade of progress, 1990-1999. Am J Clin Nutr73(2):158-71 2001 |
PubMed ID: 11157310 |
|
Goran MI, Bergman RN, Gower BA, Influence of total vs. visceral fat on insulin action and secretion in African American and white children. Obes Res9(8):423-31 2001 |
PubMed ID: 11500522 |
|
Goran MI, Gower BA, Longitudinal study on pubertal insulin resistance. Diabetes50(11):2444-50 2001 |
PubMed ID: 11679420 |
|
Herd SL, Gower BA, Dashti N, Goran MI, Body fat, fat distribution and serum lipids, lipoproteins and apolipoproteins in African-American and Caucasian-American prepubertal children. Int J Obes Relat Metab Disord25(2):198-204 2001 |
PubMed ID: 11410820 |
|
Huang TT, Johnson MS, Figueroa-Colon R, Dwyer JH, Goran MI, Growth of visceral fat, subcutaneous abdominal fat, and total body fat in children. Obes Res9(5):283-9 2001 |
PubMed ID: 11346669 |
|
Johnson MS, Figueroa-Colon R, Huang TT, Dwyer JH, Goran MI, Longitudinal changes in body fat in African American and Caucasian children: influence of fasting insulin and insulin sensitivity. J Clin Endocrinol Metab86(7):3182-7 2001 |
PubMed ID: 11443186 |
|
Li C, Johnson MS, Goran MI, Effects of low birth weight on insulin resistance syndrome in caucasian and African-American children. Diabetes Care24(12):2035-42 2001 |
PubMed ID: 11723079 |
|
Sun M, Gower BA, Bartolucci AA, Hunter GR, Figueroa-Colon R, Goran MI, A longitudinal study of resting energy expenditure relative to body composition during puberty in African American and white children. Am J Clin Nutr73(2):308-15 2001 |
PubMed ID: 11157329 |
|
Goran MI, Visceral fat in prepubertal children: Influence of obesity, anthropometry, ethnicity, gender, diet, and growth. Am J Human Biol11(2):201-207 1999 |
PubMed ID: 11533944 |
Split Ratio |
1:4 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not Inactivated |
Substrate |
None specified |
Subcultivation Method |
dilution - add fresh medium |
Supplement |
- |
|
|