AG09387
LCL from B-Lymphocyte
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Family Member
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3
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Relation to Proband
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mother
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The donor is the normal mother of a Down's syndrome child. The culture was initiated on 2/17/87 by transformation of lymphocytes with Epstein Barr virus. The culture grows in suspension and the cell morphology is spherical. The karyotype is 46,XX; normal diploid female. The affected child of this donor is AG09394. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Shi J, Tan P, Han D, Zhang R, Li J, Zhang R, Non-invasive prenatal screening for foetal trisomy: An assessment of reliability and reporting Clinical biochemistry100:71-77 2022 |
| PubMed ID: 34843730 |
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| Lee J, Bignone PA, Coles LS, Liu Y, Snyder E, Larocca D, Induced pluripotency and spontaneous reversal of cellular aging in supercentenarian donor cells Biochemical and biophysical research communications100:71-77 2020 |
| PubMed ID: 32115145 |
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| Rava RP, Srinivasan A, Sehnert AJ, Bianchi DW, Circulating Fetal Cell-Free DNA Fractions Differ in Autosomal Aueuploidies and Monosomy X Clin Chem100:71-77 2013 |
| PubMed ID: 24046201 |
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| Allinson SL, Dianova II, Dianov GL, DNA polymerase beta is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts. EMBO J20(23):6919-26 2001 |
| PubMed ID: 11726527 |
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| Podlutsky AJ, Dianova II, Podust VN, Bohr VA, Dianov GL, Human DNA polymerase beta initiates DNA synthesis during long-patch repair of reduced AP sites in DNA. EMBO J20(6):1477-82 2001 |
| PubMed ID: 11250913 |
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| Dianov GL, Thybo T, Dianova II, Lipinski LJ, Bohr VA, Single nucleotide patch base excision repair is the major pathway for removal of thymine glycol from DNA in human cell extracts. J Biol Chem275:11809-13 2000 |
| PubMed ID: 10766805 |
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| Prasad R, Dianov GL, Bohr VA, Wilson SH, FEN1 stimulation of DNA polymerase beta mediates an excision step in mammalian long patch base excision repair. J Biol Chem275:4460-6 2000 |
| PubMed ID: 10660619 |
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| Balajee AS, Machwe A, May A, Gray MD, Oshima J, Martin GM, Nehlin JO, Brosh R, Orren DK, Bohr VA, The Werner syndrome protein is involved in RNA polymerase II transcription. Mol Biol Cell10:2655-68 1999 |
| PubMed ID: 10436020 |
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| Dianov G, Bischoff C, Sunesen M, Bohr VA, Repair of 8-oxoguanine in DNA is deficient in Cockayne syndrome group B cells. Nucleic Acids Res27(5):1365-8 1999 |
| PubMed ID: 9973627 |
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| Dianov GL, Prasad R, Wilson SH, Bohr VA, Role of DNA polymerase beta in the excision step of long patch mammalian base excision repair. J Biol Chem274:13741-3 1999 |
| PubMed ID: 10318775 |
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| Dianov G, Bischoff C, Piotrowski J, Bohr VA, Repair pathways for processing of 8-oxoguanine in DNA by mammalian cell extracts. J Biol Chem273:33811-6 1998 |
| PubMed ID: 9837971 |
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| Balajee AS, May A, Dianov GL, Friedberg EC, Bohr VA, Reduced RNA polymerase II transcription in intact and permeabilized Cockayne syndrome group B cells. Proc Natl Acad Sci U S A94(9):4306-11 1997 |
| PubMed ID: 9113985 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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