AG08056
Fibroblast from Skin, Abdomen
Description:
CANIS
ORDER: CARNIVORA
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Animals |
|
Biopsy Source
|
Abdomen
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Abdomen
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Canis familiaris
|
|
Common Name
|
dog
|
|
Remarks
|
|
| PDL at Freeze |
1 |
| Passage Frozen |
1 |
| |
| Genbank |
COX1 |
| Remarks |
Beagle breed. The skin biopsy was taken post-mortem on 3/5/85. The culture was initiated using explants of minced skin tissue. Cell morphology is fibroblast-like. A matching lung fibroblast culture from this dog is AG08057. |
| Al Adhami H, Bardet AF, Dumas M, Cleroux E, Guibert S, Fauque P, Acloque H, Weber M, A comparative methylome analysis reveals conservation and divergence of DNA methylation patterns and functions in vertebrates BMC biology20:70 2022 |
| PubMed ID: 35317801 |
| Cumulative PDL at Freeze |
1 |
| Passage Frozen |
1 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|