AG07127
Fibroblast from Skin, Arm
Description:
MACACA MULATTA
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Nonhuman Primates
Animals |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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42,XX
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Species
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Macaca mulatta
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Common Name
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rhesus monkey
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Remarks
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| PDL at Senescence |
63 |
| PDL at Freeze |
5 |
| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Genbank |
COX1 |
| Remarks |
Indian strain of rhesus macaque; the skin biopsy was obtained 10/31/83, ante-mortem, from the inner aspect of the upper left arm. |
| Steinert S, White DM, Zou Y, Shay JW, Wright WE, Telomere biology and cellular aging in nonhuman primate cells. Exp Cell Res272(2):146-52 2002 |
| PubMed ID: 11777339 |
| PDL at Senescence |
63 |
| Cumulative PDL at Freeze |
5 |
| Passage Frozen |
3 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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