AG02602
Fibroblast from Skin, Abdomen
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Abdomen
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Abdomen
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5.18 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The biopsy was taken post-mortem on 8/4/77. The culture was initiated using explants of minced skin tissue. The cell morphology is fibroblastlike. The karyotype is 46,XX with 14% of the cells examined showing random chromosome loss/gain and 6% showing random chromosomal aberrations. A matched lung-derived fibroblast culture from same donor is AG02603. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Wu HF, Huang CW, Daga KR, Marklein RA, Ivanova N, Zeltner N, Human pluripotent stem cell-derived functional sympathetic neurons express ACE2 and RAAS components: a framework for studying the effect of COVID-19 on sympathetic responsiveness Clinical autonomic research : official journal of the Clinical Autonomic Research Society: 2022 |
| PubMed ID: 35091835 |
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| Gailit J, Clark RA, Studies in vitro on the role of alpha v and beta 1 integrins in the adhesion of human dermal fibroblasts to provisional matrix proteins fibronectin, vitronectin, and fibrinogen. J Invest Dermatol106:102-8 1996 |
| PubMed ID: 8592057 |
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| Querfurth HW, Wijsman EM, St George-Hyslop PH, Selkoe DJ, Beta APP mRNA transcription is increased in cultured fibroblasts from the familial Alzheimer's disease-1 family. Brain Res Mol Brain Res28(2):319-37 1995 |
| PubMed ID: 7723630 |
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| Greenhaw GA, Hebert A, Duke-Woodside ME, Butler IJ, Hecht JT, Cleaver JE, Thomas GH, Horton WA, Xeroderma pigmentosum and Cockayne syndrome: overlapping clinical and biochemical phenotypes. Am J Hum Genet50(4):677-89 1992 |
| PubMed ID: 1372469 |
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| McNearney T, Ballard L, Seya T, Atkinson JP, Membrane cofactor protein of complement is present on human fibroblast, epithelial, and endothelial cells. J Clin Invest84:538-45 1989 |
| PubMed ID: 2474570 |
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| Azzarone B, Macieira-Coelho A, Role of cytoskeletal elements in the retractile activity of human skin fibroblasts. Exp Cell Res155:299-304 1984 |
| PubMed ID: 6541590 |
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| Chapman ML, Zaun MR, Gracy RW, Changes in NAD levels in human lymphocytes and fibroblasts during aging and in premature aging syndromes. Mech Ageing Dev21:157-67 1983 |
| PubMed ID: 6223188 |
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| Tollefsbol TO, Zaun MR, Gracy RW, Increased lability of triosephosphate isomerase in progeria and Werner's syndrome fibroblasts. Mech Ageing Dev20:93-101 1982 |
| PubMed ID: 7176709 |
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| Tu AS, Robertson RS, Callahan MM, Thayer PS, Caffeine-metabolizing capability of primary rat hepatocytes, Chinese hamster V79 cells and normal human fibroblasts in culture. Mutat Res95:475-92 1982 |
| PubMed ID: 7121495 |
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| Rohme D, Ageing and the fusion sensitivity potential of human cells in culture: relation to tissue origin, donor age, and in vitro culture level and condition. Mech Ageing Dev16:241-53 1981 |
| PubMed ID: 7278394 |
| Cumulative PDL at Freeze |
13.18 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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