AG14149
Fibroblast from Skin, Scalp
Description:
ALZHEIMER DISEASE; AD
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Alzheimer's Disease |
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Biopsy Source
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Scalp
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Scalp
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Race
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White
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Family Member
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7
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Relation to Proband
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sibling
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The donor was clinically affected with Alzheimer's disease. Donor's parent and 3 siblings were affected. Donor is the product of a consanguineous marriage. The biopsy was taken 6 hours post-mortem and the culture was initiated using collagenase digestion of skin tissue. The cell morphology is fibroblast-like. The karyotype is normal with 6% of the cells examined showing random chromosome loss/gain and 4% showing random chromosomal aberrations. Culture was frozen at passage 7 and the PDL is unknown. Donor's affected siblings are AG10956B, AG10984A, and AG11001. A lymphoblast culture from same donor sampled before onset of symptoms is AG10987. Autopsy confirmed Alzheimer's disease. Donor was heterozygous for the 4 allele of apolipoprotein E. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Martin ES, Martin SE, Edelsohn L, Borgaonkar DS, Studies in a large family with late-onset Alzheimer disease (LOAD). Alzheimer Dis Assoc Disord11:163-70 1997 |
| PubMed ID: 9305502 |
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| Borgaonkar DS, Schmidt LC, Martin SE, Kanzer MD, Edelsohn L, Growdon J, Farrer LA, Linkage of late-onset Alzheimer's disease with apolipoprotein E type 4 on chromosome 19 [letter] Lancet342:625 1993 |
| PubMed ID: 8102761 |
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| Borgaonkar, Studies in a family with late on-set familial Alzheimers disease. Am J Hum Genet51:A337 (1992):625 1992 |
| PubMed ID: 8102761 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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