AG12120
Endothelial from Aorta, thoracic
Description:
SUS
ORDER: CETARTIODACTYLA
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Animals |
|
Biopsy Source
|
Aorta, thoracic
|
|
Cell Type
|
Endothelial
|
|
Tissue Type
|
Artery
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Endothelial from Aorta, thoracic
|
|
Ethnicity
|
YORKSHIRE
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Sus scrofa
|
|
Common Name
|
pig
|
|
Remarks
|
|
| PDL at Freeze |
9 |
| Passage Frozen |
5 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
Yorkshire breed. The thoracic aorta was removed immediately post-mortem on 3/12/92. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. The cell morphology is polygonal. The culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). The culture was grown on gelatin-coated flasks and the medium was supplemented with ECGS (0.02 mg/ml) and heparin (0.05 mg/ml). Culture medium: HF12 with 10% plasma-derived donor horse serum with supplements. The HF12 medium used should be endotoxin-free. Other cultures from this pig are AG12077 and AG12119. The karyotype is 38,XX with 2% of the cells examined showing random chromosome loss. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Cumulative PDL at Freeze |
9 |
| Passage Frozen |
5 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% plasma-derived horse serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
|