AG08526
LCL from B-Lymphocyte
Description:
ALZHEIMER DISEASE; AD
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
German Alzheimer Disease |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Ethnicity
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GERMAN
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Family Member
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19
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Relation to Proband
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V-31
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Confirmation
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Clinical summary/Case history
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ISCN
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45,XY,dic(13;19)(p11.3;q13.4){8}/ 46,XY{92}
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
This donor was initially diagnosed as Parkinson's disease because he exhibited a slowing of movements. Within 1 year he developed problems with attention span and memory. Further progression of intellectual impairment necessitated assistance with all activities of daily living. Treatment with anti-Parkinsonian medications did not help motor or intellectual function. EEG revealed general slowing. CSF exam was remarkable for an increase in protein concentration. CT scan demonstrated widened sulci and ventricular dilatation. Autopsy confirmed Alzheimer's disease. The culture was initiated on 11/05/85 by transformation of lymphocytes with Epstein Barr virus. The cells grow in suspension and their morphology is spherical. The culture is a mosaic with karyotype: 45,XY,dic(13;19)(p11.3;q13.4)/46,XY; unbalanced; 8%/92% with 6% of the cells examined showing random chromosome loss and 12% showing random chromosomal aberrations. A skin fibroblast culture from same donor is AG08527A. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Frommelt P, Schnabel R, Kuhne W, Nee LE, Polinsky RJ, Familial Alzheimer disease: a large, multigeneration German kindred. Alzheimer Dis Assoc Disord5:36-43 1991 |
| PubMed ID: 2025423 |
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| St George-Hyslop PH, Tanzi RE, Polinsky RJ, Haines JL, Nee L, Watkins PC, Myers RH, Feldman RG, Pollen D, Drachman D, et al, The genetic defect causing familial Alzheimer's disease maps on chromosome 21. Science235:885-90 1987 |
| PubMed ID: 2880399 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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