AG08504
Smooth muscle from Aorta, thoracic
Description:
BOS
ORDER: CETARTIODACTYLA
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Animals |
|
Biopsy Source
|
Aorta, thoracic
|
|
Cell Type
|
Smooth muscle
|
|
Tissue Type
|
Artery
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Smooth muscle from Aorta, thoracic
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Bos taurus
|
|
Common Name
|
cow
|
|
Remarks
|
|
| PDL at Senescence |
102 |
| PDL at Freeze |
12 |
| Passage Frozen |
4 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Angus breed. The age of the donor is estimated. The thoracic aorta was removed immediately post-mortem on 10/22/85. Strips of the medial layer were removed, minced, and used as explants to initiate the culture. Cell morphology is fibroblast-like. The culture tested positive for alpha-smooth muscle actin and negative for the endothelial cell-specific von Willebrand factor (immunofluorescence assays). An endothelial cell culture from this cow is AG08503. |
| Brown XQ, Ookawa K, Wong JY, Evaluation of polydimethylsiloxane scaffolds with physiologically-relevant elastic moduli: interplay of substrate mechanics and surface chemistry effects on vascular smooth muscle cell response Biomaterials26:3123-9 2004 |
| PubMed ID: 15603807 |
| |
| Sugitani H, Wachi H, Murata H, Sato F, Mecham RP, Seyama Y, Characterization of an in vitro model of calcification in retinal pigmented epithelial cells Journal of atherosclerosis and thrombosis10:48-56 2003 |
| PubMed ID: 12621165 |
| PDL at Senescence |
102 |
| Cumulative PDL at Freeze |
12 |
| Passage Frozen |
4 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|