AG08132
Endothelial from Aorta, thoracic
Description:
BOS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| PDL at Senescence |
122 |
| PDL at Freeze |
16 |
| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Holstein breed. The thoracic aorta was taken 4 hours post-mortem on 4/18/85. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. The cell morphology is polygonal. The karyotype is 60,XX; normal diploid female. The culture was grown on gelatin-coated flasks and the medium was supplemented with ECGS (0.02 mg/ml) and heparin (0.05 mg/ml). The culture tested positive for the endothelial cell-specific von Willebrand factor and negative for smooth muscle cell alpha-actin (immunofluorescence assays). Culture medium: HF12 15I with supplements. Other cultures from this cow are AG08130, AG08131A and AG08133A. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Pieper GM, Dondlinger LA, Antioxidant pyrrolidine dithiocarbamate prevents defective bradykinin-stimulated calcium accumulation and nitric oxide activity following exposure of endothelial cells to elevated glucose concentration. Diabetologia41(7):806-12 1998 |
| PubMed ID: 9686922 |
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| Ziegelstein RC, Corda S, Pili R, Passaniti A, Lefer D, Zweier JL, Fraticelli A, Capogrossi MC, Initial contact and subsequent adhesion of human neutrophils or monocytes to human aortic endothelial cells releases an endothelial intracellular calcium store. Circulation90(4):1899-907 1994 |
| PubMed ID: 7923678 |
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| Pili R, Corda S, Passaniti A, Ziegelstein RC, Heldman AW, Capogrossi MC, Endothelial cell Ca2+ increases upon tumor cell contact and modulates cell-cell adhesion. J Clin Invest92(6):3017-22 1993 |
| PubMed ID: 8254056 |
| PDL at Senescence |
122 |
| Cumulative PDL at Freeze |
16 |
| Passage Frozen |
4 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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