AG07217
Fibroblast from Lung, Lung
Description:
WI-38 - SV40 VIRUS-TRANSFORMED FETAL LUNG FIBROBLAST
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Specially Characterized Fibroblasts |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Simian Virus 40
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Sample Source
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Fibroblast from Lung, Lung
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line WI-38 VA13, subline 2RA is a post-crisis SV40 virus-transformed derivative of WI-38, and was derived 3/64 by infecting WI-38 cells at passage 23 with SV40 virus. Loss of contact inhibition occurred 48 days post-infection and crisis occurred 20 weeks post-infection. T-antigen positive cells are present and the karyotype is hyperdiploid. G6PD isoenzyme is type B. The culture was frozen at passage 257. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Perry JJ, Yannone SM, Holden LG, Hitomi C, Asaithamby A, Han S, Cooper PK, Chen DJ, Tainer JA, WRN exonuclease structure and molecular mechanism imply an editing role in DNA end processing Nature structural & molecular biology13:414-22 2006 |
| PubMed ID: 16622405 |
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| Gosslau A, Chen M, Ho CT, Chen KY, A methoxy derivative of resveratrol analogue selectively induced activation of the mitochondrial apoptotic pathway in transformed fibroblasts Br J Cancer92(3):513-21 2005 |
| PubMed ID: 15668717 |
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| Marciniak RA, Cavazos D, Montellano R, Chen Q, Guarente L, Johnson FB, A novel telomere structure in a human alternative lengthening of telomeres cell line. Cancer Res65(7):2730-7 2005 |
| PubMed ID: 15805272 |
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| Xin H, Pereira-Smith OM, Choubey D, Role of IFI 16 in cellular senescence of human fibroblasts. Oncogene23(37):6209-17 2004 |
| PubMed ID: 15208661 |
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| Pignolo RJ, Francis MK, Rotenberg MO, Cristofalo VJ, Putative role for EPC-1/PEDF in the G0 growth arrest of human diploid fibroblasts Journal of cellular physiology195:12-20 2003 |
| PubMed ID: 12599204 |
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| Lu J, Ho CH, Ghai G, Chen KY, Resveratrol analog, 3,4,5,4'-tetrahydroxystilbene, differentially induces pro-apoptotic p53/Bax gene expression and inhibits the growth of transformed cells but not their normal counterparts. Carcinogenesis22(2):321-8 2001 |
| PubMed ID: 11181455 |
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| Hisama FM, Chen YH, Meyn MS, Oshima J, Weissman SM, WRN or telomerase constructs reverse 4-nitroquinoline 1-oxide sensitivity in transformed Werner syndrome fibroblasts. Cancer Res60(9):2372-6 2000 |
| PubMed ID: 10811112 |
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| Lu J, Ho CT, Ghai G, Chen KY, Differential effects of theaflavin monogallates on cell growth, apoptosis, and Cox-2 gene expression in cancerous versus normal cells. Cancer Res60(22):6465-71 2000 |
| PubMed ID: 11103814 |
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| Luberto C, Yoo DS, Suidan HS, Bartoli GM, Hannun YA, Differential effects of sphingomyelin hydrolysis and resynthesis on the activation of NF-kappa B in normal and SV40-transformed human fibroblasts. J Biol Chem275:14760-6 2000 |
| PubMed ID: 10799564 |
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| Luberto C, Hannun YA, Sphingomyelin synthase, a potential regulator of intracellular levels of ceramide and diacylglycerol during SV40 transformation. Does sphingomyelin synthase account for the putative phosphatidylcholine-specific phospholipase C? J Biol Chem273:14550-9 1998 |
| PubMed ID: 9603970 |
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| Dimri GP, Campisi J, Altered profile of transcription factor-binding activities in senescent human fibroblasts. Exp Cell Res212:132-40 1994 |
| PubMed ID: 8174635 |
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| Saito H, Moses RE, Immortalization of Werner syndrome and progeria fibroblasts Experimental cell research192:373-9 1991 |
| PubMed ID: 1671011 |
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| Sun Y, Moses RE, Reactivation of psoralen-reacted plasmid DNA in Fanconi anemia, xeroderma pigmentosum, and normal human fibroblast cells. Somat Cell Mol Genet17:229-38 1991 |
| PubMed ID: 2047939 |
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| McNearney T, Ballard L, Seya T, Atkinson JP, Membrane cofactor protein of complement is present on human fibroblast, epithelial, and endothelial cells. J Clin Invest84:538-45 1989 |
| PubMed ID: 2474570 |
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| Takahashi S, Nakagawa S, Zeydel M, Bhargava G, Ammonia production by IMR-90 fibroblast cultures: effects of ammonia on glutathione, gamma-glutamyl transpeptidase, lysosomal enzymes, and cell division. J Cell Physiol125:107-14 1985 |
| PubMed ID: 2864350 |
| Split Ratio |
1:7 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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