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AG06939 Fibroblast from Skin, Arm

Description:

PAN TROGLODYTES
ORDER: PRIMATES

Affected:

No

Sex:

Male

Age:

9 YR (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Images
  • Culture Protocols

Overview

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Repository NIA Aging Cell Culture Repository
Subcollection Nonhuman Primates
Animals
Biopsy Source Arm
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Sample Source Fibroblast from Skin, Arm
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Pan troglodytes
Common Name chimpanzee
Remarks The skin biopsy was taken ante-mortem on 7/25/83 from the medial surface of the left arm.

Distribution of Nonhuman Primate samples is limited to academic and not-for-profit groups and institutions within the United States. Limitations on the number and type of samples may apply.

Characterizations

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PDL at Freeze 12
Passage Frozen 7
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Chromosome Analysis
 
Genbank Lipoprotein Lp(a)
 
Genbank Plasminogen
 
Genbank Apolipoprotein B
 
Genbank Nuclear Receptor Subfamily 1, Group H, Member 2

Phenotypic Data

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Remarks The skin biopsy was taken ante-mortem on 7/25/83 from the medial surface of the left arm.

Publications

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Babcock M, Yatsenko S, Stankiewicz P, Lupski JR, Morrow BE, AT-rich repeats associated with chromosome 22q112 rearrangement disorders shape human genome architecture on Yq12 Genome research17:451-60 2007
PubMed ID: 17284672
 
Han K, Sen SK, Wang J, Callinan PA, Lee J, Cordaux R, Liang P, Batzer MA, Genomic rearrangements by LINE-1 insertion-mediated deletion in the human and chimpanzee lineages. Nucleic Acids Res33(13):4040-52 2005
PubMed ID: 16034026
 
Ho HJ, Ray DA, Salem AH, Myers JS, Batzer MA, Straightening out the LINEs: LINE-1 orthologous loci. Genomics85(2):201-7 2005
PubMed ID: 15676278
 
Fortna A, Kim Y, MacLaren E, Marshall K, Hahn G, Meltesen L, Brenton M, Hink R, Burgers S, Hernandez-Boussard T, Karimpour-Fard A, Glueck D, McGavran L, Berry R, Pollack J, Sikela JM, Lineage-specific gene duplication and loss in human and great ape evolution. PLoS Biol2(7):E207 2004
PubMed ID: 15252450
 
Otieno AC, Carter AB, Hedges DJ, Walker JA, Ray DA, Garber RK, Anders BA, Stoilova N, Laborde ME, Fowlkes JD, Huang CH, Perodeau B, Batzer MA, Analysis of the human Alu Ya-lineage. J Mol Biol342(1):109-18 2004
PubMed ID: 15313610
 
Morrish TA, Gilbert N, Myers JS, Vincent BJ, Stamato TD, Taccioli GE, Batzer MA, Moran JV, DNA repair mediated by endonuclease-independent LINE-1 retrotransposition. Nat Genet31(2):159-65 2002
PubMed ID: 12006980
 
Conte RA,Samonte RV,Verma R, Localization of subtelomeric sequences of human chromosomes 1q, 11p, 13q, and 16q in the higher primates. Genetica105:31-4 1999
PubMed ID: 10483091
 
Conte RA,Samonte RV,Verma R, Evolutionary divergence of the oncogenes GLI, HST and INT2. Heredity81 ( Pt 1):10-3 1998
PubMed ID: 9720300
 
Tarzami ST,Kringstein AM,Conte RA,Verma R, Unique genomic sequences in human chromosome 16p are conserved in the great apes. Mol Gen Genet253:512-4 1997
PubMed ID: 9037113
 
Janczewski DN, Goldman D, O'Brien SJ, Molecular genetic divergence of orang utan (Pongo pygmaeus) subspecies based on isozyme and two-dimensional gel electrophoresis. J Hered81:375-87 1990
PubMed ID: 2230076
 
Ledbetter DH, Ledbetter SA, Nussbaum RL, Implications of fragile X expression in normal males for the nature of the mutation. Nature324:161-3 1986
PubMed ID: 3785381

External Links

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dbSNP dbSNP ID: 10127

Images

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View karyotype 

Culture Protocols

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Cumulative PDL at Freeze 12
Passage Frozen 7
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Medium MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent
Serum 10% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA
Supplement -
Pricing
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Non-profit:
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NIA Grantees:
$0.00USD
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