AG05809
Fibroblast from Skin, Skin
Description:
ALZHEIMER DISEASE; AD
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Alzheimer's Disease |
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Biopsy Source
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Skin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Skin
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Race
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White
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Ethnicity
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JEWISH
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Family Member
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2
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Relation to Proband
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sister
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
6.52 |
| Passage Frozen |
6 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The donor is clinically affected with moderate dementia of insidious onset. Three sibs died with autopsy proven Alzheimer's disease. The culture was initiated from explants of minced skin tissue. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XX/47,XX,+21; unbalanced; 56%/44%. The culture is frozen at PDL 14. A skin fibroblast culture from donor's affected sister is AG05810D. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Joshi AU, Van Wassenhove LD, Logas KR, Minhas PS, Andreasson KI, Weinberg KI, Chen CH, Mochly-Rosen D, Aldehyde dehydrogenase 2 activity and aldehydic load contribute to neuroinflammation and Alzheimer's disease related pathology Acta neuropathologica communications7:190 2019 |
| PubMed ID: 31829281 |
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| Khan TK, Alkon DL, An internally controlled peripheral biomarker for Alzheimer's disease: Erk1 and Erk2 responses to the inflammatory signal bradykinin Proceedings of the National Academy of Sciences of the United States of America103:13203-7 2006 |
| PubMed ID: 16920798 |
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| Mazzola JL, Sirover MA, Subcellular alteration of glyceraldehyde-3-phosphate dehydrogenase in Alzheimer's disease fibroblasts Journal of neuroscience research71:279-85 2002 |
| PubMed ID: 12503091 |
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| Mazzola JL, Sirover MA, Reduction of glyceraldehyde-3-phosphate dehydrogenase activity in Alzheimer's disease and in Huntington's disease fibroblasts. J Neurochem76(2):442-9 2001 |
| PubMed ID: 11208907 |
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| Etcheberrigaray E, Gibson GE, Alkon DL, Molecular mechanisms of memory and the pathophysiology of Alzheimer's disease. Ann N Y Acad Sci747:245-55 1994 |
| PubMed ID: 7847674 |
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| Ito E, Oka K, Etcheberrigaray R, Nelson TJ, McPhie DL, Tofel-Grehl B, Gibson GE, Alkon DL, Internal Ca2+ mobilization is altered in fibroblasts from patients with Alzheimer disease. Proc Natl Acad Sci U S A91:534-8 1994 |
| PubMed ID: 8290560 |
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| Etcheberrigaray R, Ito E, Oka K, Tofel-Grehl B, Gibson GE, Alkon DL, Potassium channel dysfunction in fibroblasts identifies patients with Alzheimer disease. Proc Natl Acad Sci U S A90:8209-13 1993 |
| PubMed ID: 8367484 |
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| Zebrower M, Beeber C, Kieras FJ, Characterization of proteoglycans in Alzheimer's disease fibroblasts. Biochem Biophys Res Commun184:1293-300 1992 |
| PubMed ID: 1590792 |
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| Huang HM, Toral-Barza L, Thaler H, Tofel-Grehl B, Gibson GE, Inositol phosphates and intracellular calcium after bradykinin stimulation in fibroblasts from young, normal aged and Alzheimer donors. Neurobiol Aging12:469-73 1991 |
| PubMed ID: 1770982 |
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| Ueda K, Cole G, Sundsmo M, Katzman R, Saitoh T, Decreased adhesiveness of Alzheimer's disease fibroblasts: is amyloid beta-protein precursor involved? Ann Neurol25:246-51 1989 |
| PubMed ID: 2729915 |
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| Van Huynh T, Cole G, Katzman R, Huang KP, Saitoh T, Reduced protein kinase C immunoreactivity and altered protein phosphorylation in Alzheimer's disease fibroblasts. Arch Neurol46:1195-9 1989 |
| PubMed ID: 2684108 |
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| Peterson C, Ratan RR, Shelanski ML, Goldman JE, Altered response of fibroblasts from aged and Alzheimer donors to drugs that elevate cytosolic free calcium. Neurobiol Aging9:261-6 1988 |
| PubMed ID: 3398992 |
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| Kinsella TJ, Dobson PP, Fornace AJ Jr, Barrett SF, Ganges MB, Robbins JH, Alzheimer's disease fibroblasts have normal repair of N-methyl-N'-nitro- N-nitrosoguanidine-induced DNA damage determined by the alkaline elution technique. Biochem Biophys Res Commun149:355-61 1987 |
| PubMed ID: 3426578 |
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| Sims NR, Finegan JM, Blass JP, Altered metabolic properties of cultured skin fibroblasts in Alzheimer's disease. Ann Neurol21:451-7 1987 |
| PubMed ID: 3109316 |
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| Peterson C, Goldman JE, Alterations in calcium content and biochemical processes in cultured skin fibroblasts from aged and Alzheimer donors. Proc Natl Acad Sci U S A83:2758-62 1986 |
| PubMed ID: 3458236 |
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| Peterson C, Ratan RR, Shelanski ML, Goldman JE, Cytosolic free calcium and cell spreading decrease in fibroblasts from aged and Alzheimer donors. Proc Natl Acad Sci U S A83:7999-8001 1986 |
| PubMed ID: 3464016 |
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| Li JC, Kaminskas E, Deficient repair of DNA lesions in Alzheimer's disease fibroblasts. Biochem Biophys Res Commun129:733-8 1985 |
| PubMed ID: 2409969 |
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| Sims NR, Finegan JM, Blass JP, Altered glucose metabolism in fibroblasts from patients with Alzheimer's disease [letter] N Engl J Med313:638-9 1985 |
| PubMed ID: 4022053 |
| Cumulative PDL at Freeze |
6.52 |
| Passage Frozen |
6 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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