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AG05352 Fibroblast from Skin, Thigh

Description:

ATELES GEOFFROYI
ORDER: PRIMATES

Affected:

No

Sex:

Male

Age:

3 DA (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIA Aging Cell Culture Repository
Subcollection Nonhuman Primates
Animals
Biopsy Source Thigh
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Sample Source Fibroblast from Skin, Thigh
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 34,XY
Species Ateles geoffroyi
Common Name black-handed spider monkey
Remarks Black-handed spider monkey.

Distribution of Nonhuman Primate samples is limited to academic and not-for-profit groups and institutions within the United States. Limitations on the number and type of samples may apply.

Characterizations

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PDL at Freeze 16
Passage Frozen 10
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis
 
Genbank COX1

Phenotypic Data

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Remarks Black-handed spider monkey.

Publications

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Diehl WE, Stansell E, Kaiser SM, Emerman M, Hunter E, Identification of Post-entry Restrictions to Mason-Pfizer Monkey Virus Infection in New World Monkey Cells Journal of virology82(22):11140-50 2008
PubMed ID: 18799582
 
Sawyer SL, Wu LI, Emerman M, Malik HS, Positive selection of primate TRIM5alpha identifies a critical species-specific retroviral restriction domain. Proc Natl Acad Sci U S A102(8):2832-7 2005
PubMed ID: 15689398
 
Song B, Gold B, O'Huigin C, Javanbakht H, Li X, Stremlau M, Winkler C, Dean M, Sodroski J, The B302(SPRY) domain of the retroviral restriction factor TRIM5alpha exhibits lineage-specific length and sequence variation in primates Journal of virology79:6111-21 2005
PubMed ID: 15857996
 
Qian Y, Jin L, Su B, Construction and characterization of bacterial artificial chromosome library of black-handed spider monkey (Ateles geoffroyi) Genome / National Research Council Canada = Génome / Conseil national de recherches Canada47:239-45 2004
PubMed ID: 15060576
 
Ejima Y, Yang L, Trans mobilization of genomic DNA as a mechanism for retrotransposon-mediated exon shuffling. Hum Mol Genet12(11):1321-8 2003
PubMed ID: 12761047
 
Steinert S, White DM, Zou Y, Shay JW, Wright WE, Telomere biology and cellular aging in nonhuman primate cells. Exp Cell Res272(2):146-52 2002
PubMed ID: 11777339
 
Schempp W, Weber B, Muller G, Mammalian sex-chromosome evolution: a conserved homoeologous segment on the X and Y chromosomes in primates. Cytogenet Cell Genet50:201-5 1989
PubMed ID: 2805817

External Links

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dbSNP dbSNP ID: 10096

Culture Protocols

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Cumulative PDL at Freeze 16
Passage Frozen 10
Split Ratio 1:8
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent
Serum 10% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA
Supplement -
Pricing
Academic &
Non-profit:
$0.00USD
NIA Grantees:
$0.00USD
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