AG04447
Fibroblast from Skin, Arm
Description:
GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
GRC |
|
Biopsy Source
|
Arm
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Arm
|
|
Race
|
White
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
46,XY/46,XY,t(4;9)(4pter>4q33::9q12> 9qter;9pter>9q12::4q33>4qter)
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Senescence |
40 |
| PDL at Freeze |
9 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Remarks |
The biopsy site is the mesial aspect of the mid-upper left arm, taken antemortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XY/46,XY,t(4;9)(4pter>4q33::9q12>9qter;9pter>9q12::4q33>4qter); balanced; 90%/10%. A culture initiated from a biopsy taken 15 years later from this same donor is AG12602. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Miyoshi N, Oubrahim H, Chock PB, Stadtman ER, Age-dependent cell death and the role of ATP in hydrogen peroxide-induced apoptosis and necrosis Proceedings of the National Academy of Sciences of the United States of America103:1727-31 2006 |
| PubMed ID: 16443681 |
| |
| Colige A, Nusgens B, Lapiere CM, Response to epidermal growth factor of skin fibroblasts from donors of varying age is modulated by the extracellular matrix. J Cell Physiol145:450-7 1990 |
| PubMed ID: 1703167 |
| |
| Schneider EL, Monticone R, Smith J, Braunschweiger K, Roberts T, Skin fibroblast cultures derived from members of the Baltimore longitudinal study: a new resource for studies of cellular aging. Cytogenet Cell Genet31:40-6 1981 |
| PubMed ID: 7307581 |
| PDL at Senescence |
40 |
| Cumulative PDL at Freeze |
9 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Medium |
Eagle's MEM with Hank's BSS, with 26mM Hepes; ambient CO2 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|