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AG04401 Fibroblast from Skin, Skin

Description:

ALZHEIMER DISEASE; AD

Affected:

Yes

Sex:

Female

Age:

53 YR (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIA Aging Cell Culture Repository
Subcollection Alzheimer's Disease
Biopsy Source Skin
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Sample Source Fibroblast from Skin, Skin
Race White
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks The donor is a clinically affected member of a family with Alzheimer's disease. She had a 5 year history of severe dementia, decreased shortterm memory and decreased word finding ability. A brain biopsy confirmed the diagnosis. Fibroblast cultures from 2 affected sibs are AG04400A and AG04402A. The skin biopsy was taken ante-mortem. The culture was initiated on 10/17/80 using explants of minced skin tissue. The cell morphology is fibroblast-like. The karyotype is 46,XX with 6% of the cells examined showing random chromosome loss/gain. Culture grows slowly. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Characterizations

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PDL at Freeze 6.37
Passage Frozen 15
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 

Phenotypic Data

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Remarks The donor is a clinically affected member of a family with Alzheimer's disease. She had a 5 year history of severe dementia, decreased shortterm memory and decreased word finding ability. A brain biopsy confirmed the diagnosis. Fibroblast cultures from 2 affected sibs are AG04400A and AG04402A. The skin biopsy was taken ante-mortem. The culture was initiated on 10/17/80 using explants of minced skin tissue. The cell morphology is fibroblast-like. The karyotype is 46,XX with 6% of the cells examined showing random chromosome loss/gain. Culture grows slowly. The legacy karyotype description shown in this Remark may not be representative of the current available product.

Publications

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Iannuzzi F, Frisardi V, Annunziato L, Matrone C, Might Fibroblasts from Patients with Alzheimer's Disease Reflect the Brain Pathology? A Focus on the Increased Phosphorylation of Amyloid Precursor Protein Tyr Brain sciences11: 2020
PubMed ID: 33466666
 
Khan TK, Alkon DL, An internally controlled peripheral biomarker for Alzheimer's disease: Erk1 and Erk2 responses to the inflammatory signal bradykinin Proceedings of the National Academy of Sciences of the United States of America103:13203-7 2006
PubMed ID: 16920798
 
Ibarreta D, Duchen M, Ma D, Qiao L, Kozikowski AP, Etcheberrigaray R, Benzolactam (BL) enhances sAPP secretion in fibroblasts and in PC12 cells. Neuroreport10(5):1035-40 1999
PubMed ID: 10321481
 
Jenkins EC, Ye L, Gu H, Wisniewski HM, Mitotic index and Alzheimer's disease. Neuroreport9(17):3857-61 1998
PubMed ID: 9875718
 
Parshad RP, Sanford KK, Price FM, Melnick LK, Nee LE, Schapiro MB, Tarone RE, Robbins JH, Fluorescent light-induced chromatid breaks distinguish Alzheimer disease cells from normal cells in tissue culture. Proc Natl Acad Sci U S A93:5146-50 1996
PubMed ID: 8643543
 
Kim CS, Han YF, Etcheberrigaray R, Nelson TJ, Olds JL, Yoshioka T, Alkon DL, Alzheimer and beta-amyloid-treated fibroblasts demonstrate a decrease in a memory-associated GTP-binding protein, Cp20. Proc Natl Acad Sci U S A92:3060-4 1995
PubMed ID: 7708775
 
Etcheberrigaray E, Gibson GE, Alkon DL, Molecular mechanisms of memory and the pathophysiology of Alzheimer's disease. Ann N Y Acad Sci747:245-55 1994
PubMed ID: 7847674
 
Ito E, Oka K, Etcheberrigaray R, Nelson TJ, McPhie DL, Tofel-Grehl B, Gibson GE, Alkon DL, Internal Ca2+ mobilization is altered in fibroblasts from patients with Alzheimer disease. Proc Natl Acad Sci U S A91:534-8 1994
PubMed ID: 8290560
 
Etcheberrigaray R, Ito E, Oka K, Tofel-Grehl B, Gibson GE, Alkon DL, Potassium channel dysfunction in fibroblasts identifies patients with Alzheimer disease. Proc Natl Acad Sci U S A90:8209-13 1993
PubMed ID: 8367484
 
Huang HM, Gibson GE, Altered beta-adrenergic receptor-stimulated cAMP formation in cultured skin fibroblasts from Alzheimer donors. J Biol Chem268:14616-21 1993
PubMed ID: 8100816
 
Kinsella TJ, Dobson PP, Fornace AJ Jr, Barrett SF, Ganges MB, Robbins JH, Alzheimer's disease fibroblasts have normal repair of N-methyl-N'-nitro- N-nitrosoguanidine-induced DNA damage determined by the alkaline elution technique. Biochem Biophys Res Commun149:355-61 1987
PubMed ID: 3426578
 
Sims NR, Finegan JM, Blass JP, Altered metabolic properties of cultured skin fibroblasts in Alzheimer's disease. Ann Neurol21:451-7 1987
PubMed ID: 3109316
 
Peterson C, Goldman JE, Alterations in calcium content and biochemical processes in cultured skin fibroblasts from aged and Alzheimer donors. Proc Natl Acad Sci U S A83:2758-62 1986
PubMed ID: 3458236
 
Peterson C, Ratan RR, Shelanski ML, Goldman JE, Cytosolic free calcium and cell spreading decrease in fibroblasts from aged and Alzheimer donors. Proc Natl Acad Sci U S A83:7999-8001 1986
PubMed ID: 3464016
 
Sims NR, Blass JP, Phosphofructokinase activity in fibroblasts from patients with Alzheimer's disease and age- and sex-matched controls. Metab Brain Dis1:83-90 1986
PubMed ID: 2977422
 
Peterson C, Gibson GE, Blass JP, Altered calcium uptake in cultured skin fibroblasts from patients with Alzheimer's disease [letter] N Engl J Med312:1063-5 1985
PubMed ID: 3982463
 
Sims NR, Finegan JM, Blass JP, Altered glucose metabolism in fibroblasts from patients with Alzheimer's disease [letter] N Engl J Med313:638-9 1985
PubMed ID: 4022053

External Links

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dbSNP dbSNP ID: 10078
NCBI GTR 104300 ALZHEIMER DISEASE; AD
OMIM 104300 ALZHEIMER DISEASE; AD
Omim Description ALZHEIMER DISEASE, FAMILIAL; FAD
  ALZHEIMER DISEASE; AD
  PRESENILE AND SENILE DEMENTIA

Culture Protocols

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Cumulative PDL at Freeze 20.37
Passage Frozen 15
Split Ratio 1:2
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagles Minimum Essential Medium with Earle's salts:Dulbecco's modified MEM with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not inactivated
Supplement -
Pricing
Commercial:
$257.00USD
Academic &
Non-profit:
$103.00USD
NIA Grantees:
$47.00USD
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