Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently Healthy Collection
Biopsy Source Inguinal area
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Race Black
Country of Origin USA
Relation to Proband proband
Confirmation Karyotypic analysis and Case history
Species Homo sapiens
Common Name Human
Remarks Subject is clinically normal; skin biopsy from inguinal region; original culture shows karyotype of 46,XY (6% of cells show random chromosome loss and 2% are tetraploid);
PDL at Freeze 5.82
Passage Frozen 5
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Chromosome Analysis
 
Remark Subject is clinically normal; skin biopsy from inguinal region; original culture shows karyotype of 46,XY (6% of cells show random chromosome loss and 2% are tetraploid);
Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19: 2018
PubMed ID: 30567591
 
Sohar I, Sleat DE, Jadot M, Lobel P, Biochemical characterization of a lysosomal protease deficient in classical late infantile neuronal ceroid lipofuscinosis (LINCL) and development of an enzyme-based assay for diagnosis and exclusion of LINCL in human specimens and animal models. J Neurochem73:700-11 1999
PubMed ID: 10428067
No data is available
Cumulative PDL at Freeze 10.8
Passage Frozen 5
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 15% fetal bovine serum Not inactivated
Substrate None specified