Description:
TRANSGENIC MOUSE ES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals
Stem Cell Collection |
| Protocols |
Protocol PDF |
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Cell Type
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Stem cell
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Tissue Type
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Embryonic
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Transformant
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pZhcSfi
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ISCN
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40,dic(X;15)(F5;A1),Y[1]/40,XY,i(1)(A1)[1]/40,XY,del(1)(E1)[1]/40,XY,t(14;17)(C2;E5)[1]/40,XY[16]
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| Passage Frozen |
27 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
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| mouse embryonic stem cell |
The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| Passage Frozen |
27 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Gelatin + Feeder Layer |
| Supplement |
- |
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