Karyotype analysis is a laboratory technique where the chromosomes from one cell are visualized under a microscope to investigate the total number and structure of the chromosomes. Cells are obtained from an individual and are viewed during metaphase, a stage in cell division when our chromosomes are condensed. The chromosomes are stained with a dye (Giemsa), resulting in a banding pattern of light and dark stripes, known as G-banding. The patterns are specific, allowing us to identify each chromosome.

Karyotype analysis will:

• Organize all chromosomes by homology, size and shape.
• Provide an overview of the whole genome.
• Detect both numerical and structural chromosomal aberrations (overall resolution is 5 Mega bases (Mb); breakpoint resolution is 5 to 15 Mb).

Through karyotype analysis, you will obtain the following information about your cell line(s):

• Species identification
• Index of genomic stability
• Validation of normal diploid karyotypes
• Numerical chromosomal abnormalities
  • Monoploidy, polyploidy, etc.
  • Monosomy, trisomy, etc.
• Structural chromosomal rearrangements
  • Deletions
  • Duplications
  • Translocations
  • Inversions
  • Ring chromosomes and marker chromosomes
  • Isochromosome