Fluorescence in Situ Hybridization (FISH)
Aquarius Monosomy 1p36 probes have been applied to investigate a human cell line. Detection of two green signals for 1qter probes yet only one red signal for 1p36 indicates a deletion of 1p36 at one copy of chromosome 1. [ish del(1)(p36.33)(SKI-,D1S3739+)]
Fluorescence in situ hybridization (FISH) detects the presence, copy numbers and/or location of a DNA sequence within a set of metaphase chromosomes or an interphase nucleus. It is an important molecular cytogenetic technique widely used in diagnosing various genetic disorders and/or to further confirm chromosomal abnormalities found by other assays such as karyotyping and array-based comparative genomic hybridization (aCGH).
FISH, also known as whole chromosome painting, is valuable in defining rearrangements among different chromosomes and the origin of ring and marker chromosomes that are not clearly deciphered by karyotype analysis.
The majority of probes utilized in our FISH assays are analytic specific reagents (ASRs) purchased from vendors in the field. Customers may provide probes; however, we strongly recommend proper validation of the reagents prior to sample analysis. Our lab at Coriell is skilled in probe design and validation testing, and we are always happy to assist researchers in developing the best approach to analysis.
Mouse B and T lymphocyte attenuator (BTLA) specific probes were applied to test a mouse embryonic stem cell line. [ish 16B5(BTLAx2)]
A human cell line with a translocation between one chromosome 4 (red signals) and one chromosome 6 (green signals) was tested with a whole chromosomal painting assay, note the chromosomal exchange (red and green within the same chromosomes). [ish t(4;6)(wcp4+,wcp6+;wcp4+,wcp6+)]
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