Since conventional cytogenetics methods gather data on a series of single cells, their greatest strength is the ability to detect chromosomal abnormalities at low frequencies. G-banded karyotype analysis is the primary tool for assuring that cell lines retain normal diploid chromosome complements and therefore, essentially normal genomes within the resolution limit of 5 to 10 megabases. Many more thorough analytical techniques are also available for use in cytogenetic analyses and will provide a much more in-depth view at chromosomal alterations within genomes.

Since 1999, the Coriell Cytogenetics Laboratory has analyzed thousands of human and mouse embryonic stem cell (ESC) lines and induced pluripotent stem cell (iPSC) lines for researchers in academia, pharmaceuticals, and beyond.

Our services also offer assurance to transgenic animal facilities by confirming that no apparent chromosomal abnormalities are present within ESC or iPSC lines being used to generate chimeras, as those abnormalities may compromise the chimeras germline transmission, fertility, or the ability to produce otherwise normal progeny. As an example, even a low frequency of trisomy 8 cells or trisomy 11 cells in mouse ESCs is detrimental since they confer a selective advantage and can overgrow cultures in just a small number of passages.