Coriell Institute for Medical Research

Steven J. Madore

Steven J. Madore, Ph.D., is an Associate Professor and Director of the Molecular Biology Group. He received a B.S. degree in Microbiology from the University of Massachusetts, Amherst and a Ph.D. in Cell and Developmental Biology from the State University of New York at Stony Brook in 1991 where he studied cis-acting regulatory elements in the apolipoprotein E gene. Upon completing his graduate degree he joined the laboratory of Dr. Bryan Cullen at Duke University Medical Center from 1991 – 1994 as a postdoctoral fellow investigating the molecular mechanisms of HIV-1 gene regulation. While at Duke Dr. Madore demonstrated experimentally that prior to binding to the HIV-1 promoter- proximal TAR RNA stem loop the viral trans-activating protein Tat binds to and recruits a cellular co-factor necessary for the activation of viral gene expression. In 1995 he accepted a faculty appointment as Research Investigator in the Dermatology Research Division at the University of Michigan Medical Center in Ann Arbor where he studied gene regulatory mechanisms linked to dermal photodamage. Dr. Madore then joined the Molecular Biology Department at the Parke-Davis/Pfizer Research and Development facility in Ann Arbor, MI in 1998 as the Expression Profiling Group Leader. In this role he directed the use of Affymetrix gene expression profiling technology in support of drug discovery programs in Oncology, Inflammation, Neuroscience, and Dermatology. In 2007 he accepted a position in the Discovery Technology Group of GlaxoSmithKline Research and Development in Collegeville, PA as Manager of the Transcript Biology Laboratory. In this role he led the use of comprehensive RNA expression profiling technologies in critical support of drug discovery efforts in Oncology and Metabolic Diseases.

Dr. Madore was appointed the Director of Molecular Biology at Coriell in December of 2008 and now oversees all processing and quality assessment of nucleic acid for the institute. This includes the development of protocols for the large-scale preparation of DNA and RNA samples and establishing quality control procedures. He also supervises and coordinates the activities of staff involved in the molecular characterization of genomic DNA including microsatellite analysis and gender determination using PCR. The Molecular Biology Group also performs whole genome amplification, Mycoplasma testing for the Coriell Cell Culture Laboratory and Single Nucleotide Polymorphisms (SNP) analysis. Dr. Madore conducts applied research aimed at improving and streamlining the methods employed by the Repository to improve efficiency. He also oversees the documentation of all of the relevant processes and activities of the Molecular Biology Group for entry into the Coriell database.

Dr. Madore is also the principal investigator for the Breast Cancer Family Registry biobank and coordinates the receipt, processing, and distribution of genomic DNA samples to cancer researchers throughout the world. His research interests include the molecular mechanisms regulating gene expression including the role of miRNAs in disease processes. Dr. Madore teaches undergraduates at Rowan University, served as an editorial board member for the Journal of Biological Chemistry from 2001-2006 and is a member of the American Association for the Advancement of Science.

Peer-reviewed Articles

Mutlib, A., P. Jiang, J. Atherton, L. Obert, S. Kostrubsky, S.J. Madore, and S. Nelson (2006)
Identification of potential genomic biomarkers of hepatotoxicity caused by reactive metabolites of N-methylformamide: Application of stable isotope labeled compounds in toxicogenomic studies. Chem. Res. Toxicol., 19:10, 1270-83.

Gieseg, M.A., M. Z. Man, N. A. Gorski, S.J. Madore, E. P. Kaldjian, and W.R. Leopold (2004)
The influence of tumor size and environment on gene expression in commonly used tumor lines. BMC Cancer 2004, 4:35.

Robinson, M., P. Jiang, J. Cui, J. Li, Y. Wang, M. Swaroop, S.J. Madore, T.S. Lawrence, and Y. Sun (2003)
Global Genechip profiling to identify genes responsive to p53-induced growth arrest and apoptosis in human lung carcinoma cells. Cancer Biology and Therapy 2:4, 406-415.

Gieseg, M.A., T. Cody, M.Z. Man, S.J. Madore, M.A. Rubin and E.P. Kaldjian (2002)
Expression profiling of human renal carcinomas with functional taxonomic analysis. BMC Bioinformatics 2002, 3:26.

Wells, J., C.R. Graveel, S.M. Bartley, S.J. Madore, and P.J. Farnum (2002)
The identification of E2F1-specific target genes. Proc. Natl. Acad. Sci. USA, 99:3890-3895.

Hu, G.K., S.J. Madore, B. Moldover, T. Jatkoe, D. Balaban, J.D. Thomas, and Y. Wang (2001)
Predicting splice variants from Affymetrix GeneChip data. Genome Research, 11:1237-1245.

Gravel, C., T. Jatkoe, S.J. Madore, A.L. Holt, and P.J. Farnum (2001)
Expression profiling and identification of novel genes in hepatocellular carcinomas. Oncogene, 20:2704-2712.

Kane, M.D., T. Jatkoe, C. Stumpf, J.D. Thomas, and S.J. Madore (2000)
Assessment of the sensitivity and specificity of oligo-probe (50mer) microarrays. Nucleic Acid Research, 28:4552-4557.

Swaroop M., Y. Wang, P. Miller, H. Duan, T. Jatkoe, S.J. Madore, Y. Sun (2000)
Yeast homologue of human SAG/ROC2/Rbx2/Hrt2 is essential for cell growth, but not for germination: chip profiling implicates its role in cell cycle regulation. Oncogene, 19:2855-2866.

Bogerd, H.P., R.A. Fridell, S.J. Madore, and B.R. Cullen (1995)
Identification of a novel cellular co-factor for the Rev/Rex class of retroviral regulatory proteins. Cell, 82:485-494.

Madore, S.J. and B.R. Cullen (1995)
Functional similarities between HIV-1 tat and DNA sequence-specific transcriptional activators. Virology, 206:1150-1154.

Blair, W.S., H.P. Bogerd, S.J. Madore, and B.R. Cullen (1994)
Mutational analysis of the transcriptional activation domain of relA: Identification of a highly synergistic minimal acidic activation module. Molecular and Cellular Biology, 14:7226-7234.

Madore, S.J., L.S. Tiley, M.H. Malim, and B.R. Cullen (1994)
Sequence requirements for rev multimerization in vivo. Virology, 202:186-194.

Luo, Y., S.J. Madore, T.G. Parslow, B.R. Cullen, and B.M. Peterlin (1993)
Functional analysis of interactions between tat and the trans-activation response element of human immunodeficiency virus type 1 in cells. J. Virology, 67:5617-5622.

Madore, S.J. and B.R. Cullen (1993)
Genetic analysis of the co-factor requirement for human immunodeficiency virus type 1 tat function. J. Virology, 67:3703-3711.

Tiley, L.S., S.J. Madore, M.H. Malim, and B.R. Cullen (1992)
The VP16 transcription activation domain is functional when targeted to a promoter-proximal RNA sequence. Genes & Development, 6:2077-2087.

Madore, S.J., E.D. Wieben, G.R. Kunkel, and T. Pederson (1984)
Precursors of U4 small nuclear RNA. J. Cell Biology, 99:1140-1144.

Madore, S.J., E.D. Wieben, and T. Pederson (1984)
Eucaryotic small ribonucleoproteins. Anti-La human autoantibodies react with U1 RNA-protein complexes. J. Biological Chemistry, 259:1929-1933.

Madore, S.J., E.D. Wieben, and T. Pederson (1984)
Intracellular site of U1 small nuclear RNA processing and ribonucleoprotein assembly. J. Cell Biology, 98:188-192.

Wieben, E.D., S.J. Madore, and T. Pederson (1983)
U1 snRNP studied by in vitro assembly. J. Cell Biology, 96:1751-1755.

Wieben, E.D., S.J. Madore, and T. Pederson (1983)
Protein binding sites are conserved in U1 small nuclear RNA from insects and mammals. Proc. Natl. Acad. Sci. USA, 80:1217-1220.

Book Chapters

Kane, M.D., A.A. Dombowski, and S.J. Madore (2001)
The emerging utility of oligonucleotide microarrays in Gene Cloning and Expression Technologies, Edited by M.P. Weiner and Q. Lu, Eaton Publishing, Westborough, MA.